| 低温冰箱冷冻对人体组织拉曼光谱的影响 |
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| 引用本文： | 刘刚,张林,俞帆,刘剑虹,孙世中.低温冰箱冷冻对人体组织拉曼光谱的影响[J].光谱实验室,2004,21(5):838-841. |
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| 作者姓名： | 刘刚 张林 俞帆 刘剑虹 孙世中 |
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| 作者单位： | 云南师范大学物理系,昆明市,650092;昆明医学院附属第一医院病理检验科,昆明市,650031;云南大学实验中心,昆明市,650091;云南师范大学分析测试中心,昆明市,650092 |
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| 基金项目： | 云南省教育厅科学研究基金 (No0 2 ZY0 72 )，云南省高校联合测试基金资助项目 |
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| 摘 要： | 研究了低温冰箱冷冻保存时间对人体甲状腺组织、子宫肌瘤组织的拉曼光谱的影响。组织用磷酸缓冲生理液浸泡 ,于 - 4℃保存 (未冻 ) 1 0 h,测试拉曼光谱 ;测后将组织样品浸入磷酸缓冲生理液 ,置于低温冰箱 - 30℃保存。分别于冷冻几天、十几天和六十天后 ,取出解冻测试拉曼光谱。结果表明 ,冷冻保存 1 0天内对组织的拉曼光谱没有明显的影响。该结果对拉曼光谱研究人体组织具有指导意义
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| 关 键 词： | 人体组织 拉曼光谱 冷冻 |
| 文章编号： | 1004-8138(2004)05-0838-04 |
| 修稿时间： | 2004年2月6日 |
Effects of Frozen Time on the Raman Spectra of Human Tissues |
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| LIU Gang ZHANG Lin,a,YU Fan,b,LIU Jian Hong,c,SUN Shi Zhong,c.Effects of Frozen Time on the Raman Spectra of Human Tissues[J].Chinese Journal of Spectroscopy Laboratory,2004,21(5):838-841. |
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| Authors: | LIU Gang ZHANG Lin a YU Fan b LIU Jian Hong c SUN Shi Zhong c |
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| Abstract: | The effects of frozen time on the Raman spectra of human thyroid and uterine muscle benign tumor tissues at 514.5nm excitation were studied.The samples were kept in phosphate buffered saline,without freezing,and tested in 10h after the surgical resection.The same specimen was then frozen in cryogenic refrigerator,and examined at intervals from several days to 60 days.The results show that the Raman spectra of human tissues were not observed obvious changes by storage in cryogenic refrigerator within 10 days.These studies could be utilized to guide future experimental design during Raman spectroscopy of biological tissues. |
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| Keywords: | Human Tissue Raman Spectroscopy Freeze |
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