| 高分离度快速液相色谱法测定牛黄解毒片中黄芩苷的含量 |
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| 引用本文： | 尔西丁·买买提,葛亮,王昕宇,田树革.高分离度快速液相色谱法测定牛黄解毒片中黄芩苷的含量[J].光谱实验室,2010,27(4):1322-1324. |
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| 作者姓名： | 尔西丁·买买提 葛亮 王昕宇 田树革 |
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| 作者单位： | 1. 新疆医科大学公共卫生学院,乌鲁木齐市新医路393号,830011
2. 新疆名医名方与特色方剂学重点实验室,乌鲁木齐市,830011 |
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| 摘 要： | 采用高分离度快速液相色谱(RRLC)测定牛黄解毒片中黄芩苷的含量。色谱柱为Zorbax XDB-C18(4.6×50mm,1.8μm),流动相为甲醇-水-磷酸(45∶55∶0.2),检测波长315nm,流速0.5mL.min-1,柱温为30℃。黄芩苷在0.15—1.35μg(r=0.9999)范围内峰面积与浓度呈良好的线性关系,加样回收率为100.41%,RSD=1.42%。采用高分离度快速液相色谱(RRLC)测定复方牛黄解毒片中黄芩苷的含量,缩短了保留时间,提高了效率,降低了成本。本方法简便、准确,结果稳定。
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| 关 键 词： | 高分离度快速液相色谱 牛黄解毒片 黄芩苷 |
Determination of Content of Baicalin in Niuhuang Jiedu Tablet by RRLC |
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| ER Xiding-Maimaiti,GE Liang,WANG Xin-Yu,TIAN Shu-Ge.Determination of Content of Baicalin in Niuhuang Jiedu Tablet by RRLC[J].Chinese Journal of Spectroscopy Laboratory,2010,27(4):1322-1324. |
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| Authors: | ER Xiding-Maimaiti GE Liang WANG Xin-Yu TIAN Shu-Ge |
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| Abstract: | The content of baicalin in Niuhuang Jiedu tablet was determined by RRLC.Zorbax XDB-C18（4.6×50mm,1.8μm）was as the colum,with the methanol-water-phosphoric acid（45：55：0.2） as mobile phase,at the flow rate of 0.5mL·min-1,and detection wavelength of 315nm.The linear range was 0.15—1.35μg,r=0.9999,the average recovery was 100.41% with RSD of 1.42%.The retention time of baicalin by RRLC is short.The method is efficient,cheap,simple,accuracy and stable. |
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| Keywords: | RRLC Niuhuang Jiedu Tablet Baicalin |
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