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槐枝中总黄酮的含量测定
引用本文:赵超,陈华国,靳凤云,周欣,龚小见.槐枝中总黄酮的含量测定[J].光谱实验室,2010,27(1):188-191.
作者姓名:赵超  陈华国  靳凤云  周欣  龚小见
作者单位:1. 贵州师范大学天然药物质量控制研究中心贵阳市宝山北路116号,550001;贵州省山地环境信息系统与生态环境保护重点实验室,贵阳市,550001
2. 贵阳中医学院,贵阳市,550002
基金项目:贵州省中小企业局平台建设项目黔中小发[2008]76号;;贵州省发展和改革委员会黔科合带帽字[2008]5005号
摘    要:采用索氏提取法提取槐枝中的总黄酮,芦丁为对照,分光光度法测定其含量。样品浓度在16.104—96.624μg.mL-1范围内线性关系良好,相关系数r=0.9996。该方法简便、准确,可用于槐枝中总黄酮的含量测定。

关 键 词:槐枝  黄酮  芦丁  分光光度法

Determination of Total Flavonoids from the Branch of Sophora aponica L.
ZHAO Chao,CHEN Hua-Guo,JIN Feng-Yun,ZHOU Xin,GONG Xiao-Jian.Determination of Total Flavonoids from the Branch of Sophora aponica L.[J].Chinese Journal of Spectroscopy Laboratory,2010,27(1):188-191.
Authors:ZHAO Chao  CHEN Hua-Guo  JIN Feng-Yun  ZHOU Xin  GONG Xiao-Jian
Institution:The Research Center for Quality Control of Natural Medicine;Guizhou Normal University;Guiyang 550001;P.R.China;Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment;P.R.China;Guiyang College of TCM;Guiyang 550002;P.R.China
Abstract:The total flavonoids of the branch of Sophora aponica L.were determined by spectrophotometry with the Soxhlet's extraction method and rutin as the quality control standard.The relationship between the absorbance and the concentration of sample was linear in the range of 16.10~4-96.624μg·mL~(-1),and the correlation coefficient was 0.9996.This method is simple,accurate and can be applied to determine the content of total flavonoids from the branch of Sophora aponica L.
Keywords:The Branch of Sophora aponica L    Flavonoids  Rutin  Spectrophotometry  
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