| 黄绿蜜环菌菌丝体水提物多糖含量测定 |
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| 引用本文： | 党军,王瑛,陶燕铎,邵赞,梅丽娟,王启兰.黄绿蜜环菌菌丝体水提物多糖含量测定[J].光谱实验室,2011,28(6):2836-2840. |
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| 作者姓名： | 党军 王瑛 陶燕铎 邵赞 梅丽娟 王启兰 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所 西宁市西关大街59号810008;中国科学院研究生院 北京市100049
2. 中国科学院西北高原生物研究所 西宁市西关大街59号810008 |
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| 基金项目： | 国家科技支撑计划项目(2007BAI45B00) |
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| 摘 要： | 采用苯酚-硫酸法测定了黄绿蜜环菌菌丝体水提物中多糖的含量。测定波长492nm,葡萄糖质量浓度与吸光度的回归方程为A=0.051C-0.1150,R2=1.0000,其平均回收率为102.22%,相对标准偏差为3.54%（n=6）。测得黄绿蜜环菌菌丝体水提物多糖含量为31.21%。
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| 关 键 词： | 黄绿蜜环菌菌丝体 多糖 苯酚-硫酸法 |
Determination of Polysaccharide in Armillarialuteo-rivens Mycelium Extract |
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| DANG Jun,WANG Ying,TAO Yan-Duo,SHAO Yun,MEI Li-Juan,WANG Qi-Lan.Determination of Polysaccharide in Armillarialuteo-rivens Mycelium Extract[J].Chinese Journal of Spectroscopy Laboratory,2011,28(6):2836-2840. |
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| Authors: | DANG Jun WANG Ying TAO Yan-Duo SHAO Yun MEI Li-Juan WANG Qi-Lan |
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| Institution: | DANG Juna,b WANG Yinga,b TAO Yan-Duoa SHAO Yuna MEI Li-Juana WANG Qi-Lanaa(Northwest Institute of Plateau Biology Chinese Academy of Sciences,Xining 810008,P.R.China)b(Graduate University of Chinese Academy of Sciences,Beijing 100049,P.R.China) |
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| Abstract: | The content of polysaccharides in Armillarialuteo-rivens mycelium extract was measured by phenol-sulfuric acid method.Wavelength for measurement was 492nm.The linear relationship of absorbance of glucose with its concentration was good with regression equation A=0.051C-0.1150(R2=1.0000),average recovery of glucose was 102.22% with RSD of 3.54%(n=6).The contents of polysaccharides in Armillarialuteo-rivens mycelium extract was 31.21%. |
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| Keywords: | Armillarialuteo-rivens Mycelium Polysaccharide Phenol-Sulfuric Acid Method |
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