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臧红T-Fenton体系荧光光谱法测定黄山贡菊/金菊的抗氧化活性
引用本文:徐金明,陈欢.臧红T-Fenton体系荧光光谱法测定黄山贡菊/金菊的抗氧化活性[J].光谱实验室,2011,28(5):2455-2459.
作者姓名:徐金明  陈欢
作者单位:黄山学院化学系 安徽省黄山市屯溪区西海路39号245041
摘    要:臧红T能产生特征荧光,其激发波长和发射波长分别为264nm和577nm.酸性介质中,Fenton体系(H2O2-Fe2+)产生的羟自由基能氧化臧红T使其荧光猝灭,而许多花茶的提取物可部分清除溶液中的羟自由基使其荧光猝灭程度降低,据此建立了一种测定黄山贡菊和金菊的抗氧化活性的新方法.本实验采用单因素实验法,对羟自由基体系...

关 键 词:臧红T  荧光分析法  黄山贡菊  抗氧化活性

Determination of Antioxidant Activity of Huangshan Chrysanthemum/Golden Aster by Fluorometry with Safranine T-Fenton System
XU Jin-Ming,CHEN Huan.Determination of Antioxidant Activity of Huangshan Chrysanthemum/Golden Aster by Fluorometry with Safranine T-Fenton System[J].Chinese Journal of Spectroscopy Laboratory,2011,28(5):2455-2459.
Authors:XU Jin-Ming  CHEN Huan
Institution:XU Jin-Ming CHEN Huan(Department of Chemistry,Huangshan University,Huangshan,Anhui 245041,P.R.China)
Abstract:Characteristic fluorescence produced by safranine T was observed with its excitation and emission wavelength at 264nm and 577nm,respectively.Fluorescence intensity of safranine T was quenched by the hydroxyl free radical produced by Fenton system in acidic medium.However,this quenching effect can be alleviated by some extractants of flower tea,because the hydroxyl free radical can be partially cleaned.Based on this principle,a fluorometry method was developed for the determination of the antioxidant activit...
Keywords:Safranine T  Fluorescence Analysis  Huangshan Chrysanthemum  Antioxidant Activity  
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