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| 双波长分光光度法同时测定载硫酸长春新碱/姜黄素聚合物纳米粒中两种药物的含量 | |
| 引用本文： | 王海鸥,袁佩,滕永真,贾运涛,张良珂.双波长分光光度法同时测定载硫酸长春新碱/姜黄素聚合物纳米粒中两种药物的含量[J].光谱实验室,2011,28(6):2820-2824. |
| 作者姓名： | 王海鸥 袁佩 滕永真 贾运涛 张良珂 |
| 作者单位： | 1. 重庆医科大学药学院 重庆市生物化学与分子药理学重点实验室 重庆市400016;重庆医科大学生命科学院 重庆市400016 2. 重庆医科大学生命科学院 重庆市400016 3. 重庆医科大学附属儿童医院药剂科 重庆市400010 |
| 基金项目： | 国家自然科学基金项目(30770565); 重庆市科委自然科学基金资助项目(CSTC,2008BB5397); 重庆市渝中区科技计划项目资助(20100203); 重庆市卫生局课题资助(2010-2-202) |
| 摘 要： | 以聚乙二醇单甲醚-聚乳酸-乙醇酸共聚物（m PEG-PLGA）为载体材料,制备同时载带硫酸长春新碱（VCR）和与之具有协同抗肿瘤作用的姜黄素（Cur）的m PEG-PLGA纳米粒（VCR/Cur-m PEG-PLGA-NP）。建立了以等吸收双波长紫外分光光度法同时测定纳米粒中VCR和Cur含量的方法,研究显示,VCR测定波长296.5nm,等吸收波长462nm;Cur测定波长414nm;VCR在4.0—36.0μg.mL-1范围内,浓度C与差值吸光度ΔA呈良好线性相关;Cur在0.5—8.0μg.mL-1范围内,浓度C与吸光度A具有良好的线性关系。该方法简便迅速,结果准确可靠,适用于VCR/Cur-m PEG-PLGA-NP中药物含量的同时测定。 |
| 关 键 词： | 双波长 紫外分光光度法 姜黄素 硫酸长春新碱 |
Simultaneous Determination of the Two Drugs in Polymeric Nanoparticles Loaded with Vincristine Sulfate and Curcumin by Dual-Wavelength Spectrophotometry |
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| WANG Hai-Ou,YUAN Pei,TENG Yong-Zhen,JIA Yun-Tao,ZHANG Liang-Ke.Simultaneous Determination of the Two Drugs in Polymeric Nanoparticles Loaded with Vincristine Sulfate and Curcumin by Dual-Wavelength Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2011,28(6):2820-2824. | |
| Authors: | WANG Hai-Ou YUAN Pei TENG Yong-Zhen JIA Yun-Tao ZHANG Liang-Ke |
| Institution: | WANG Hai-Oua,b YUAN Peib TENG Yong-Zhenb JIA Yun-Taoc ZHANG Liang-Kea,ba(School of Pharmacy,Chongqing Medical University,Chongqing 400016,P.R.China)b(Institute of Life Sciences,P.R.China)c(Department of Pharmacy,Children's Hospital of Chongqing Medical University,Chongqing 400010,P.R.China) |
| Abstract: | mPEG-PLGA nanoparticles simultaneously loaded with vincristine sulfate(VCR) and curcumin(Cur) were prepared with polyethylene glycol momoethyl ether-polylactic acid-glycollic acid copolymer(mPEG-PLGA) as carrier material.Dual-wavelength ultraviolet spectrophotometry was employed for measuring the content of the dual agent(VCR and Cur) in VCR/Cur-mPEG-PLGA-NP.The results showed that 296.5nm and 462nm were the maximum absorption wavelength and isosbestic wavelength of VCR respectively,and 414nm was the maximu... |
| Keywords: | Dual-Wavelength Ultraviolet Spectrophotometry Curcumin Vincristine Sulfate |
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