| 仁东大蒜蛋白质的测定 |
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| 引用本文： | 周振,周能.仁东大蒜蛋白质的测定[J].光谱实验室,2011,28(5):2435-2438. |
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| 作者姓名： | 周振 周能 |
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| 作者单位： | 玉林师范学院化学与生物系天然产物研究所 广西玉林市教育中路299号537000 |
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| 基金项目： | 国家自然科学基金(No.81060360); 广西科学基金(No.2010GXNSFA013065); 广西教育厅项目(No.200911MS200) |
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| 摘 要： | 采用碱提酸沉法提取分离大蒜蛋白,最佳提取条件为浸提碱液pH=7.2,浸提最佳时间为30min,沉淀的最佳pH=4.2.采用考马斯亮蓝G-250法测定大蒜中蛋白质的含量,结果表明蛋白质-色素结合物在590nm波长下的吸光度与蛋白质含量呈正比,线性范围为1.67-16.67μg/mL.标准溶液和试样的RSD分别为0.35％...
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| 关 键 词： | 仁东大蒜 蛋白质 碱提酸沉法 |
Determination of Protein in Rendong Garlic |
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| ZHOU Zhen,ZHOU Neng.Determination of Protein in Rendong Garlic[J].Chinese Journal of Spectroscopy Laboratory,2011,28(5):2435-2438. |
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| Authors: | ZHOU Zhen ZHOU Neng |
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| Institution: | ZHOU Zhen ZHOU Neng(Institute of Natural Products,Department of Chemistry and Biology,Yulin Normal University,Yulin,Guangxi 537000,P.R.China) |
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| Abstract: | The protein in garlic was extracted and separated by alkali-extraction and acid-precipitation method.The optimal extraction conditions were given:pH of 7.2 for extract,extraction time of 30 min,pH of 4.2 for precipitation.Coomassie brilliant blue G-250(CBB) was employed to determine protein in garlic,and the conditions for determination,such as,wavelengh,the dosage of colour reagents and stable time of reaction were optimized.The content of protein was in direct ratio with the absorbance of CBB-protein comp... |
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| Keywords: | Rendong Garlic Protein Alkali-Extraction and Acid-Precipitation Method |
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