| HPLC同时测定清胃黄连丸中的4种有效成分 |
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| 引用本文： | 李宝红,吴君,邓妙丽,吴劲东.HPLC同时测定清胃黄连丸中的4种有效成分[J].光谱实验室,2011,28(5):2302-2305. |
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| 作者姓名： | 李宝红 吴君 邓妙丽 吴劲东 |
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| 作者单位： | 1. 广东医学院药学院 广东省东莞市523808
2. 广州中医药大学中药学院 广州市番禺区广州大学城外环东路232号510006 |
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| 基金项目： | 2008年广东省科技计划项目(83041) |
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| 摘 要： | 建立同时测定清胃黄连丸中栀子苷、黄芩苷、小檗碱和巴马汀含量的HPLC方法。结果表明,栀子苷、黄芩苷、小檗碱和巴马汀分别在2.07—66.2μg/mL（r=0.9997）、2.72—86.9μg/mL（r=0.9998）、2.23—71.3μg/mL（r=0.9998）、2.13—68.1μg/mL（r=0.9998）范围内与峰面积呈良好的线性关系;平均回收率分别为103.2%（RSD=3.27%）、106.7%（RSD=2.46%）、103.5%（RSD=2.13%）、95.7%（RSD=2.68%）。该方法简便可行、重现性好、能更好的控制清胃黄连丸的质量。
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| 关 键 词： | 清胃黄连丸 波长切换 栀子苷 黄芩苷 小檗碱 巴马汀 |
Simultaneous Determination of Four Active Compounds in Qingwei Huanglian Pill by HPLC |
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| LI Bao-Hong,WU Jun,DENG Miao-Li,WU Jin-Dong.Simultaneous Determination of Four Active Compounds in Qingwei Huanglian Pill by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2011,28(5):2302-2305. |
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| Authors: | LI Bao-Hong WU Jun DENG Miao-Li WU Jin-Dong |
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| Institution: | LI Bao-Hong WU Juna DENG Miao-Li WU Jin-Dong(School of Pharmaceutical,Guangdong Medical College,Dongguan,Guangdong 523808,P.R.China)a(School of Tradition Chinese Medicine,Guangzhou University of T.C.M.,Guangzhou 510006,P.R.China) |
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| Abstract: | A method for simultaneous determination of geniposide,baicalin,berberine and palmatin in Qingwei Huanglian pill by HPLC was established.There were good linear relationship between concentration and peak area in the range of 2.07—66.2μg/mL for geniposide（r=0.9997）,2.72—86.9μg/mL for baicalin（r=0.9998）,2.23—71.3μg/mL for berberine（r=0.9998）,2.13—68.1μg/mL for palmatin（r=0.9998）,respectively.The average recovery of geniposide,baicalin,berberine and palmatin were 103.2%（RSD=3.27%）,106.7%（RSD=2.46%）,103.5%（RSD=2.13%） and 95.7%（RSD=2.68%）,respectively.This method is simple with good reproducibility,and could be used for quality control of Qingwei Huanglian pill. |
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| Keywords: | Qingwei Huanglian Pill Switching Wavelength Geniposide Baicalin Berberine Palmatin |
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