| 固相萃取-HPLC快速测定血清中烟酸及其代谢产物 |
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| 引用本文： | 徐雯,梁炜,孙武平,莫道章,彭慧平,王露萍,蔡小玲,景立新,王鑫.固相萃取-HPLC快速测定血清中烟酸及其代谢产物[J].光谱实验室,2011,28(6):2882-2886. |
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| 作者姓名： | 徐雯 梁炜 孙武平 莫道章 彭慧平 王露萍 蔡小玲 景立新 王鑫 |
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| 作者单位： | 大连大学医学院医学检验系 辽宁省大连市开发区学府大街10号116622 |
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| 摘 要： | 建立血清中烟酸(NA)、烟酰胺(NM)及其代谢产物N'-甲基尼克酰胺(NMN)、2-吡啶酮(2Py)的测定方法.采用高效液相色谱法(HPLC),以氨基固相柱萃取样品,0.04mol/L KH2PO4提取洗脱,同时测定血清中NMN、NA、2Py、NM的含量.校准曲线线性范围为0.5-30μg/mL,r≥0.9986.检出...
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| 关 键 词： | 烟酸 烟酰胺 N'-甲基尼克酰胺 2-吡啶酮 |
Determination of Nicotinic Acid and Its Metabolites in Serum by High Performance Liquid Chromatography with Solid-Phase Extraction |
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| XU Wen,LIANG Wei,SUN Wu-Ping,MO Dao-Zhang,PENG Hui-Ping,WANG Lu-Ping,CAI Xiao-Ling,JING Li-Xin,WANG Xin.Determination of Nicotinic Acid and Its Metabolites in Serum by High Performance Liquid Chromatography with Solid-Phase Extraction[J].Chinese Journal of Spectroscopy Laboratory,2011,28(6):2882-2886. |
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| Authors: | XU Wen LIANG Wei SUN Wu-Ping MO Dao-Zhang PENG Hui-Ping WANG Lu-Ping CAI Xiao-Ling JING Li-Xin WANG Xin |
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| Institution: | XU Wen LIANG Wei SUN Wu-Ping MO Dao-Zhang PENG Hui-Ping WANG Lu-Ping CAI Xiao-Ling JING Li-Xin WANG Xin(Department of Clinic Laboratory Sciences,Medical College of Dalian University,Dalian,Liaoning 116622,P.R.China) |
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| Abstract: | The method for the determination of nicotinic acid（NA）,nicotinamide（NM）,and their metabolites N-methyl-2-pyridone-5-carboxamide（2Py）,N′-methylnicotinamide（NMN） in serum was established by high performance liquid chromatography（HPLC）.The samples were extracted by amino solid phase column with elution of 0.04mol/L KH2PO4.The contents of NA,NMN,2Py,NM in serum were simultaneously deteceted.The linear range of method was 0.5—30μg/mL with r≥0.9986,and limits of detection were NMN of 0.45μg/mL,NA 0.51μg/mL,2Py 0.15μg/mL,NM 0.20μg/mL,respectively.The recovery was 88.6%—105.1%.The relative standard deviation was 1.3%—7.8%.The method is fast,simple,accurate and sensitive,which meets the experimental purpose and the need. |
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| Keywords: | Nicotinic Acid Nicotinamide N′-Methylnicotinamide N-Methyl-2-Pyridone-5-Carboxamide |
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