| 氢化物发生原子荧光光谱法测定螺旋藻中无机硒和有机硒 |
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| 引用本文： | 贺与平,李维香,何素芳,熊敏,单云,刘春侠.氢化物发生原子荧光光谱法测定螺旋藻中无机硒和有机硒[J].光谱实验室,2009,26(6):1404-1408. |
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| 作者姓名： | 贺与平 李维香 何素芳 熊敏 单云 刘春侠 |
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| 作者单位： | 贺与平,何素芳,熊敏,单云,刘春侠(昆明理工大学分析测试研究中心,昆明市学府路253号,650093);李维香(云南省粮油产品质量监督检验所,昆明市,650041) |
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| 基金项目： | 云南省教育厅科学研究基金 |
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| 摘 要： | 螺旋藻试样经硝酸、高氯酸消解,原子荧光光谱法测定总硒含量;用水浸提螺旋藻试样,经环己烷萃取后,水相采用硝酸、高氯酸消解后,原子荧光光谱法测定无机硒含量,间接测定螺旋藻中有机硒含量。总硒测定精密度在1.05%—2.10%之间,加标回收率在96.6%—102.3%之间;无机硒的浸提率在95.1%—100.7%之间,测定精密度在2.37%—3.70%之间,加标回收率在93.6%—98.4%之间,检出限为0.47mg/kg,满足螺旋藻中有机硒含量的测定。
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| 关 键 词： | 有机硒 无机硒 螺旋藻 氢化物发生-原子荧光光谱法 |
Determination of Organic and Inorganic Selenium in Spirulina by Hydride Generation-Atomic Florescence Spectrophotometry |
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| HE Yu-Ping LI Wei-Xianga HE Su-Fang XIONG Min SHAN Yun LIU Chun-Xia,a.Determination of Organic and Inorganic Selenium in Spirulina by Hydride Generation-Atomic Florescence Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2009,26(6):1404-1408. |
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| Authors: | HE Yu-Ping LI Wei-Xianga HE Su-Fang XIONG Min SHAN Yun LIU Chun-Xia a |
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| Institution: | HE Yu-Ping LI Wei-Xianga HE Su-Fang XIONG Min SHAN Yun LIU Chun-Xia (Research Center for Analysis and Measurement,Kunming University of Science and Technology,Kunming 650093,P.R China) a(Yunnan Product Quality Supervision and Testing Center of Food and Oil,Kunming 650041,P.R.China) |
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| Abstract: | The total selenium in spirulina was digested by mixed acid of HNO_3 and HCIO_4 and determinated by hydride generation-atomic florescence spectrophotometry (HG-AFS); After extracting the spirulina by water and then by cyclohexane,the water phase was digested by HNO_3 and HCIO_4,and the inorganic selenium was determinated by HG-AFS,consequently,the content of organic selenium was calculated. The accuracy of total Se is 1.05%-2.10%,and the recovery is 96.6%-102.3%. The extraction rate of inorganic Se is 95.1%-100.7%, the accuracy of inorganic Se is 2.37%-3.70%, and the recovery of inorganic Se is 93. 6%-98.4%,the detection limit is 0. 47 mg/kg. This method meets the determintion of organic selenium in spirulina. |
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| Keywords: | Organic Selenium Inorganic Selenium Spirulina HG-AFS |
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