| 电化学氧化-荧光分析法测定肾上腺素 |
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| 引用本文： | 李莹,杜虹,赵常志.电化学氧化-荧光分析法测定肾上腺素[J].光谱实验室,2010,27(4):1498-1502. |
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| 作者姓名： | 李莹 杜虹 赵常志 |
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| 作者单位： | 生态化工教育部重点实验室,青岛科技大学化学与分子工程学院,山东省青岛市郑州路53号,266042 |
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| 基金项目： | 应用化学国家重点实验室基金项目 |
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| 摘 要： | 通过电化学氧化肾上腺素,使其生成羟基吲哚类荧光物质(λem=490nm),实现了药物中肾上腺素含量的电化学氧化-荧光检测。研究结果表明,荧光强度与肾上腺素浓度分别在2.00×10-7—1.00×10-6mol/L和1.00×10-6—4.00×10-5mol/L范围内呈良好的线性关系,检出限为6.70×10-8mol/L。该方法用于药物制剂中肾上腺素含量的测定,相对标准偏差小于1.88%(n=7),加标回收率为88.4%—116.6%。
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| 关 键 词： | 电化学氧化-荧光分析法 肾上腺素 |
Determination of Epinephrine by Electrooxidized-Fluorescence Analysis |
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| LI Ying,Du Hong,ZHAO Chang-Zhi.Determination of Epinephrine by Electrooxidized-Fluorescence Analysis[J].Chinese Journal of Spectroscopy Laboratory,2010,27(4):1498-1502. |
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| Authors: | LI Ying Du Hong ZHAO Chang-Zhi |
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| Institution: | (Key Laboratory of Eco-Chemical Engineering,Ministry of Education,College of Chemistry ＆ Molecular Engineering,Qingdao University of Science ＆ Technology,Qingdao,Shandong 266042,P.R.China) |
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| Abstract: | A simple and sensitive electrogenerated fluorescence method has been developed by combining electrolysis and fluoremetry for the determination of epinephrine,and its product has a characteristic fluorescence at 490 nm.Under the optimum conditions,the electrogenerated fluorescent intensity is proportional to the concentration of epinephrine in the range of 2.00×10-7—1.00×10-6mol/L and 1.00×10-6—4.00×10-5mol/L,and the detection limit is 6.70×10-8mol/L.The relative standard deviation is less than 1.88%（n=7） and the recovery is in the range of 88.4%—116.6% for the determination of epinephrine in pharmaceutical samples. |
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| Keywords: | Electrooxidize-Fluorescence Analysis Method Epinephrine |
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