高效液相色谱测定卷烟及其主流烟气中的维生素E

样品用饱和抗坏血酸乙醇溶液萃取,ZORBAX Extend-C18（5μm,4.6×150mm）色谱柱及FLD检测器在激发波长为298nm,发射波长为325nm下检测,流动相为100%甲醇。采用外标法定量,线性相关系数为0.99996,检出限为0.12μg/mL,卷烟及其主流烟气中维生素E平均回收率分别为97.0%和97.4%,RSD分别为0.70%和4.70%。研究表明：烤烟型卷烟烟丝比混合型卷烟烟丝中维生素E含量高,烤烟型卷烟主流烟气比混合型卷烟主流烟气中维生素E含量高,维生素E从卷烟烟丝到主流烟气的迁移率为7%—16%。

Determination of Vitamin E in Cigarette and Mainstream Cigarette Smoke with HPLC

The cigarette mainstream smoke wet total particulate matter (WTPM) samples and cigarette samples were extracted in ethanol using ascorbic acid as an inhibitor of the degradation of vitamin E. Vitamin E was analyzed on a ZORBAX Extend-C_(18)(5μm,4.6×150mm) column with fluorescence detector at 298nm excitation and 325nm emission. A mobile phase of methanol was used. The quantitative results were obtained with external standard calibration with linear correlative coefficient of 0.99996, and the detection limit was 0.12μg/mL on the basis of signal-to-noise ratio of 3. The method is reproducible with percent relative standard deviation of 0.70%for cigarette and 4.70% for WTPM. Average recoveries were 97.0%for cigarette and 97.4% for WTPM. Vitamin E in flue-cured tobacco is higher than in blended tobacco. The flue-cured cigarettes have the high content of vitamin E than the blended cigarettes in mainstream WTPM. The percent transfer of vitamin E from tobacco to mainstream smoke is 7%-16%.