| UPLC-ELSD法测定黄芪中的黄芪甲苷含量 |
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| 引用本文： | 杨俊,王文辉,王齐,孙晓东,朱艳琴.UPLC-ELSD法测定黄芪中的黄芪甲苷含量[J].光谱实验室,2009,26(3):484-486. |
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| 作者姓名： | 杨俊 王文辉 王齐 孙晓东 朱艳琴 |
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| 作者单位： | 昆明理工大学分析测试研究中心,昆明市学府路253号,650093 |
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| 摘 要： | 采用Waters Acquity UPLC BEH C18色谱柱（2.1mm×100mm,1.7μm）,流动相为乙腈-水（30∶70）,流速为0.2mL·min^-1,柱温30℃,用UPLC-ELSD法测定黄芪中黄芪甲苷的含量。黄芪甲苷在0.10-0.50mg·mL^-1范围内有良好的线性关系（r=0.9990）,平均回收率（n=6）为98.9%。该方法快速、准确、重复性好,为黄芪的质量控制提供了一种快速准确的检测方法。
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| 关 键 词： | 超高效液相色谱-蒸发光散射 黄芪 黄芪甲苷 |
Determination of Astragaloside Ⅳ in Radix Astragali by UPLC-ELSD |
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| YANG Jun,WANG Wen-Hui,WANG Qi,SUN Xiao-Dong,ZHU Yan-Qin.Determination of Astragaloside Ⅳ in Radix Astragali by UPLC-ELSD[J].Chinese Journal of Spectroscopy Laboratory,2009,26(3):484-486. |
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| Authors: | YANG Jun WANG Wen-Hui WANG Qi SUN Xiao-Dong ZHU Yan-Qin |
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| Institution: | Research Center for Analysis and Measurement;Kunming University of Science and Technology;Kunming;Yunnan 650093;P.R.China |
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| Abstract: | The amounts of astragaloside Ⅳ in radix astragali was determined by UPLC-ELSD with a waters acquity UPLC BEH C18 column （2. 1mm × 100mm, 1. 7μm）, aeetonitrile-water （30 ： 70） as mobile phase at a flow rate of 0.2mL · min^-1 ,and the column temperature of 30℃. The calibration curve was linear in the range of 0. 10-0. 50mg · mL^-1μg of astragaloside Ⅳ with correlation coefficient of 0. 9990. The average recovery of astragaloside Ⅳ was 98.9%. The method is simple,repeatable, and suitable for the determination of astragaloside Ⅳ in radix astragali. |
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| Keywords: | UPLC-ELSD Radix Astragali Astragaloside Ⅳ |
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