| 川桂叶中黄酮类化合物的提取与测定 |
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| 引用本文： | 李姣娟,黄克瀛,龚建良,周尽花,陈丛瑾.川桂叶中黄酮类化合物的提取与测定[J].光谱实验室,2009,26(3):530-534. |
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| 作者姓名： | 李姣娟 黄克瀛 龚建良 周尽花 陈丛瑾 |
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| 作者单位： | 1. 中南林业科技大学应用化学研究所,长沙市韶山南路498号,410004
2. 中南林业科技大学材料科学与工程学院,长沙市,410004 |
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| 基金项目： | 湖南省教育厅资助项目 |
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| 摘 要： | 采用加热回流法提取川桂叶中的黄酮类化合物,建立了川桂叶中总黄酮含量测定的光谱分析方法。结果表明：以芦丁为标准品,用NaNO2-Al（NO3）3-NaOH显色法可测定川桂叶中总黄酮的含量,该方法的精密度和准确度均较高,相对标准偏差为0.63%,方法的回收率为97.18%—102.20%。通过正交试验优化,得到提取川桂叶中黄酮类化合物的最佳工艺条件是：用体积分数为70%的乙醇作溶剂,在固液比1∶20（g/mL）、提取温度80℃的条件下提取1.5h,连续提取2次。测得川桂叶中总黄酮含量为22.53mg·g^-1。
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| 关 键 词： | 川桂叶 黄酮类化合物 提取 分光光度法 |
Extraction and Determination of Flavonoids from Cinnamomum Wilsonii Gamble Leaves |
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| LI Jiao-Juan,HUANG Ke-Ying,GONG Jian-Liang,ZHOU Jin-Hua,CHEN Cong-Jin.Extraction and Determination of Flavonoids from Cinnamomum Wilsonii Gamble Leaves[J].Chinese Journal of Spectroscopy Laboratory,2009,26(3):530-534. |
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| Authors: | LI Jiao-Juan HUANG Ke-Ying GONG Jian-Liang ZHOU Jin-Hua CHEN Cong-Jin |
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| Institution: | Applied Chemistry Institute of Central South University of Forestry and Technology;Changsha 410004;P.R.China;Material Science and Engineering College of Central South University of Forestry and Technology;P.R.China |
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| Abstract: | Flavonoids were extracted from Cinnamomum wilsonii Gamble leaves by Heating refluxing, and determined by spectrophotomertry. The NaNO3-Al （NO3）2-NaOH method is practicable to determine flavonoids content from C. wilsonii Gamble leaves with rutin as the standard. This method is higher precision and accuracy with RSD 0. 63% and recoveries 97. 18%-102.20%. The optimum extraction conditions were obtained by orthogonal experiments as follows：extracting solvent 70% （V/V） ethanol, material ratio 1 ： 20 （g/mL）, extraction temperature 80℃, extraction duration 1.5h and extracting for two times. The total flavonoids content from C. wilsonii Gamble leaves is 22. 53mg · g^-1. |
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| Keywords: | Cinnamomum Wilsonii Gamble Leaves Flavonoids Extraction Spectrophotometry |
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