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大蒜素提取检测及对肝癌细胞的灭活效果研究
引用本文:任凤莲,陈莎子,代军,纪春晓.大蒜素提取检测及对肝癌细胞的灭活效果研究[J].光谱实验室,2011,28(1):374-377.
作者姓名:任凤莲  陈莎子  代军  纪春晓
作者单位:1. 中南大学化学化工学院,长沙市岳麓区麓山南路932号,410083
2. 九江学院化学化工学院,江西省九江市,332005
基金项目:国家“863”计划项目(2009AA04Z124)
摘    要:采用溶剂提取法提取大蒜中的活性成分,探讨了不同酶解温度和大蒜捣碎粒径程度对大蒜素提取率的影响.通过正交试验,研究了硫酸钡吸光比浊法测定大蒜中大蒜素含量的最佳实验条件.在最佳实验条件下,体系的吸光度y与硫酸根离子浓度x呈线性关系:y=0.237x+0.002(r=0.9985).用MTT法对大蒜素提取浓缩液进行细胞活性检...

关 键 词:提取  大蒜素  比浊法  肝癌细胞

Extraction and Determination of Allicin and Cytotoxic Activity on Hepatoma Cells
REN Feng-Lian,CHEN Sha-Zi,DAI Juna,JI Chun-Xiao.Extraction and Determination of Allicin and Cytotoxic Activity on Hepatoma Cells[J].Chinese Journal of Spectroscopy Laboratory,2011,28(1):374-377.
Authors:REN Feng-Lian  CHEN Sha-Zi  DAI Juna  JI Chun-Xiao
Institution:REN Feng-Lian CHEN Sha-Zi DAI Jun a JI Chun-Xiao(College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,P.R.China)a(College of Chemistry and Chemical Engineering,Jiujiang University,Jiujiang,Jiangxi 332005,P.R.China)
Abstract:Solvent extraction method was used to extract allicin.The influence of various hydrolysis temperatures and mashed garlic size on the extraction rate of allicin was discussed.The conditions of BaSO4 turbidimetry for measuring the content of allicin in garlic were optimized by orthogonal method.The absorbance(y)was proportional to the concentration of SO2-4(x),y=0.237x+0.002,with a correlation coefficient of 0.9985 under optimal experimental conditions.Cell activity was determined for allicin extract by MTT m...
Keywords:Extraction  Allicin  Turbidimetry  Hepatoma Cells  
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