| 磷钼酸铵分光光度法测定茶多酚 |
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| 引用本文： | 王齐,王文辉,杨俊,孙晓东,朱艳琴,陈伟.磷钼酸铵分光光度法测定茶多酚[J].光谱实验室,2010,27(1):210-212. |
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| 作者姓名： | 王齐 王文辉 杨俊 孙晓东 朱艳琴 陈伟 |
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| 作者单位： | 昆明理工大学莲华校区分析测试研究中心,昆明市学府路253号,650000;云南省分析测试中心,昆明市,650000 |
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| 基金项目： | 昆明理工大学分析测试研究中心主任基金(2008-01) |
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| 摘 要： | 在pH=5.3的醋酸-醋酸铵(HAc-NH4Ac)介质中,茶多酚与磷钼酸铵结合形成磷钼酸酯,溶液最大吸收波长为320nm。茶多酚的浓度在0.01—0.20mg/mL范围内遵守比耳定律,其表观摩尔吸光系数为ε=1.22×105L.mol-1.cm-1。方法选择性较好,用于茶汤中总茶多酚含量的测定,所得结果与标准方法测定结果基本一致。
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| 关 键 词： | 分光光度法 茶多酚 磷钼酸铵 |
Determination of Tea Polyphenols by Ammonium Phosphomol ybdate Spectrophotometry |
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| WANG Qi,WANG Wen-Hui,YANG Jun,SUN Xiao-Dong,ZHU Yan-Qin,CHEN Wei.Determination of Tea Polyphenols by Ammonium Phosphomol ybdate Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2010,27(1):210-212. |
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| Authors: | WANG Qi WANG Wen-Hui YANG Jun SUN Xiao-Dong ZHU Yan-Qin CHEN Wei |
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| Institution: | Research Center for Analysis and Measurement;Kunming University of Science and Technology;Kunming 650000;P.R.China;Analytic and Testing Reseach Center of Yunnan;P.R.China |
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| Abstract: | In Hac-NH_4Ac at Ph=5.3 buffer solution,the tea polyphenol reacts with ammonium phosphomolybdate and forms the ester phosphomolybdate.The wavelength for the spectrophotometric determination is 320nm.Beer's law is obeyed in the range of 0.01-0.20mg/ML,and the apparent molar absorptivity ε is 1.22×10~5 L·moL~(-1)·cm~(-1).The content of tea polyphenols in the tea soup was determined. |
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| Keywords: | Spectrophotometry Tea Polyphenols Ammonium Phosphomolybdate |
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