| 脱氧雪腐镰刀菌烯醇与溶菌酶相互作用的研究 |
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| 引用本文： | 李晓梅,李玉琴,侯海峰,冀海伟,李群伟.脱氧雪腐镰刀菌烯醇与溶菌酶相互作用的研究[J].光谱实验室,2010,27(3):809-814. |
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| 作者姓名： | 李晓梅 李玉琴 侯海峰 冀海伟 李群伟 |
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| 作者单位： | 1. 泰山医学院流行病研究所,山东省泰安市长城路中段,271000
2. 泰山医学院药学院,山东省泰安市,271016 |
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| 基金项目： | 山东省自然科学基金，山东省教育厅基金 |
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| 摘 要： | 利用荧光猝灭、紫外光谱(UV)、傅里叶变换红外光谱(FTIR)和圆二色性光谱(CD)法测定反应的结合常数、主要作用力以及脱氧雪腐镰刀菌烯醇对溶菌酶二级结构的影响;并综合实验所得的热力学参数结果,确定二者间的作用力。脱氧雪腐镰刀菌烯醇与溶菌酶有较强的结合,脱氧雪腐镰刀菌烯醇对溶菌酶的荧光猝灭以氢键和范德华力为主,结合位置与色氨酸残基间的距离r为4.62nm。通过光谱法研究模拟生理条件下真菌毒素脱氧雪腐镰刀菌烯醇与溶菌酶的相互作用,为脱氧雪腐镰刀菌烯醇在体内的储存、运输及毒性机制提供一些具有指导作用的信息。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 溶菌酶 相互作用 |
Research on Interaction of Deoxynivalenol with Lysozyme |
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| LI Xiao-Mei,LI Yu-Qin,HOU Hai-Feng,JI Hai-Wei,LI Qun-Wei.Research on Interaction of Deoxynivalenol with Lysozyme[J].Chinese Journal of Spectroscopy Laboratory,2010,27(3):809-814. |
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| Authors: | LI Xiao-Mei LI Yu-Qin HOU Hai-Feng JI Hai-Wei LI Qun-Wei |
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| Institution: | LI Xiao-Mei LI Yu-Qin a HOU Hai-Feng JI Hai-Wei a LI Qun-Wei(Epidemiology Institute,Taishan Medicine College,Taian,Shandong 271000,P.R.China)a(College of Pharmacy,Taishan Medicine College,Taian,Shandong 271016,P.R.China) |
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| Abstract: | Fluorescence quenching methods,UV,FTIR and CD technique were used to determine the binding constants,drug-binding modes,and protein structure changes in the presence of deoxynivalenol.The deoxynivalenol bound to LYSO with higher binding,and the quenching reactions were the hydrogen bond and van der Waals＇ forces,and the distance between deoxynivalenol and LYSO was 4.62nm.The study was designed to examine the interaction between deoxynivalenol and LYSO under simulated physiological conditions,providing some directing information for the storage,transporation,and toxicity mechanism of the deoxynivalenol in vivo. |
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| Keywords: | Deoxynivalenol Lysozyme Interaction |
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