| 荧光光度法研究金莲橙G与牛血清白蛋白的作用 |
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| 引用本文： | 陈宁生,傅应强,张国庆,郑欣,孙丽丽.荧光光度法研究金莲橙G与牛血清白蛋白的作用[J].光谱实验室,2010,27(5):1715-1719. |
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| 作者姓名： | 陈宁生 傅应强 张国庆 郑欣 孙丽丽 |
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| 作者单位： | 安徽工程大学生化工程学院,安徽省芜湖市赭山东路,241000 |
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| 基金项目： | 安徽省科学计划研究项目，安徽省芜湖科技字2006 |
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| 摘 要： | 利用荧光光度法研究了金莲橙G与牛血清白蛋白(BSA)的相互作用。金莲橙G对牛血清白蛋白的内源荧光有显著的猝灭作用,是形成超分子复合物的静态猝灭过程。测定了金莲橙G与BSA结合常数以及其相互作用的热力学参数,并根据Ross理论,和作用过程的热力学参数确定了金莲橙G与牛血清白蛋白之间主要结合力是静电引力。由Foerster非辐射能量转移理论,推导出能量供体金莲橙G与受体BSA间的结合距离。
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| 关 键 词： | 金莲橙G 牛血清白蛋白 荧光光度法 相互作用 |
Study on the Interaction Between Tropeolin G and Bovine Serum Albumin by Fluorescence Spectroscopy |
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| CHEN Ning-Sheng,FU Ying-Qiang,ZHANG Guo-Qing,ZHENG Xin,SUN Li-Li.Study on the Interaction Between Tropeolin G and Bovine Serum Albumin by Fluorescence Spectroscopy[J].Chinese Journal of Spectroscopy Laboratory,2010,27(5):1715-1719. |
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| Authors: | CHEN Ning-Sheng FU Ying-Qiang ZHANG Guo-Qing ZHENG Xin SUN Li-Li |
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| Institution: | (College of Biochemical Engineering,Anhui Engineering University,Wuhu,Anhui 241000,P.R.China) |
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| Abstract: | The interaction between tropeolin G and bovine serum albumin（BSA）was investigated mainly by fluorescence spectroscopy.Tropeolin G can remarkably quench the fluorescence intensity of BSA,and the fluorescence quenching effect is a static quenching process with forming the supramolecular complex.The binding constants and thermodynamic parameters of interaction between tropeolin G and bovine serum albumin were studied.Basing on the theory of Ross,the main sort of binding force between tropeolin G and BSA were attributed to static-electricity gravitation,which was conformed by the calculation results of thermodynamic parameters of this process.The binding distance between tropeolin G and BSA was obtained according to Foerster＇s nonradioactive energy transfer theory. |
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| Keywords: | Tropeolin G Bovine Serum Albumin Fluorescence Spectroscopy Interaction |
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