| RP-HPLC测定罗勒中石吊兰素的含量 |
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| 引用本文： | 王绍云,曹晖,唐文华,吴林冬.RP-HPLC测定罗勒中石吊兰素的含量[J].光谱实验室,2010,27(2):780-784. |
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| 作者姓名： | 王绍云 曹晖 唐文华 吴林冬 |
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| 作者单位： | 凯里学院应用化学研究所贵州省凯里市鸭塘镇(经济开发区)开元大道3号,556011 |
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| 基金项目： | 贵州省教育厅自然科学类科研项目(2007104) |
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| 摘 要： | 建立反相-高效液相色谱法(RP-HPLC)测定罗勒(Ocimum basilicumL.)中石吊兰素的含量。用Microsorb-M V 100-5C18(4.6mm×250mm,5μm)色谱柱,甲醇:1%醋酸=86:14(V/V)为流动相,流速为0.5mL/min,检测波长为335nm。石吊兰素浓度在0.27—32.4μg/mL范围内与峰面积线性关系良好(r=0.9999),方法平均回收率大于99%(n=9)。方法简单、可靠,可应用于罗勒植物中石吊兰素的含量测定。
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| 关 键 词： | 罗勒 石吊兰素 反相一高效液相色谱法 |
Determination of Nevadensin in Ocimum Basilicum by RP-HPLC |
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| Wang Shao-Yun,Cao Hui,Tang Wen-Hua,Wu Lin-Dong.Determination of Nevadensin in Ocimum Basilicum by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2010,27(2):780-784. |
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| Authors: | Wang Shao-Yun Cao Hui Tang Wen-Hua Wu Lin-Dong |
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| Institution: | Institute Applied Chemistry of Kaili University;Guizhou;Kaili 556000;P.R.China |
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| Abstract: | An RP-HPLC method was established for the determination of Nevadensin in Ocimum basilicum L..The conditions of chromatography: Microsorb-MV100-5C_(18) column (4.6mm × 250mm, 5μm)was adopted with a mobile phase of methanol-1% acetic acid (86 : 14) ;the flow rate was set at 0.5mL/min,and the wavelength of detector was set at 335nm.The calibration curve was linear within the range of 0.27-32.4μg/mL (r= 0.9999) ,the average recovery was over 99%.The method has been successfully applied to the determination of nevadensin in Ocimum basilicum L.,and proves to be simple and reliable. |
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| Keywords: | Ocimum Basilicum L Nevadensin RP-HPLC |
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