| 光固定辣根过氧化酶修饰电极的直接电化学 |
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| 引用本文： | 高海燕,侯秀良,许芳萍,刘仁,陶华,顾伟,汪茹.光固定辣根过氧化酶修饰电极的直接电化学[J].光谱实验室,2009,26(6). |
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| 作者姓名： | 高海燕 侯秀良 许芳萍 刘仁 陶华 顾伟 汪茹 |
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| 作者单位： | 高海燕,许芳萍,刘仁,陶华,顾伟,汪茹(江南大学化学与材料工程学院,江苏省无锡市蠡湖大道1800号,214122);侯秀良(江南大学生态纺织教育部重点实验室 江苏省无锡市蠡湖大道1800号 214122) |
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| 基金项目： | 江苏省博士后科研资助计划 |
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| 摘 要： | 用聚氨酯丙烯酸酯预聚体将辣根过氧化物酶用光固定法制备了辣根过氧化物酶修饰电极.采用循环伏安法研究了该电极在没有媒介体的条件下的电化学性质,并考察了其对H_2O_2的生物电催化活性.结果表明,该电极可以在没有媒介体存在的条件下进行直接而稳定的电子转移.扫描100圈后,扫描时的峰电流仍然几乎不变;在缓冲溶液中保存30天后,其伏安响应仍能保持保存前的约95%以上,且仍保持对H_2O_2电化学还原的生物电催化作用.这为酶电极的制备及酶的固定化提出了新方法.
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| 关 键 词： | 辣根过氧化物酶修饰电极 光固定法 直接电化学 |
Direct Electrochemistry of Horseradish Peroxidase Modified Electrode Immobilized by Sunlight |
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| GAO Hai-Yan a HOU Xiu-Liang XU Fang-Ping a LIU Ren a TAO Hua a GU Wei a WANG Ru a.Direct Electrochemistry of Horseradish Peroxidase Modified Electrode Immobilized by Sunlight[J].Chinese Journal of Spectroscopy Laboratory,2009,26(6). |
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| Authors: | GAO Hai-Yan a HOU Xiu-Liang XU Fang-Ping a LIU Ren a TAO Hua a GU Wei a WANG Ru a |
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| Institution: | GAO Hai-Yan a HOU Xiu-Liang XU Fang-Ping a LIU Ren a TAO Hua a GU Wei a WANG Ru a (Key Laboratory of Science & Technology of Eco-Textile,Ministry of Education,Jiangnan University,Wuxi,Jiangsu 214122,P.R.China) (School of Chemistry and Material Engineering,Jiangsu 214122 P.R.China) |
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| Abstract: | Horseradish peroxidase(HRP)was immobilized on the surface of glassy carbon(GC).Using polyurethane acrylates(PUA) under the sunlight condition,HRP on electrode surface can undergo effective and stable direct electron transfer reaction with the formal redox potential.After continuous scanning 100 times,the peak current kept almost constant.At least 95% value of voltammetric response was remained after 30 days.The operation life of this electrode was very long due to the strong adhesion of PUA on glassy carbon... |
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| Keywords: | Horseradish Peroxidase Modified Electrode Immobilized by Sunlight Direct Electrochemistry |
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