| 薄层扫描法测定消渴丸中葛根素的含量 |
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| 引用本文： | 程庚金生,彭金年,张付远,李丹丹.薄层扫描法测定消渴丸中葛根素的含量[J].光谱实验室,2013(6):2915-2917. |
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| 作者姓名： | 程庚金生 彭金年 张付远 李丹丹 |
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| 作者单位： | [1]赣南医学院药学院,江西省赣州市章贡区医学院路1号341000 [2]江西环境工程职业学院林业与环境学院,江西省赣州市章贡区湖边341000 |
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| 摘 要： | 以氯仿-甲醇-乙酸乙酯-水（3∶3∶4∶1）为展开剂,在硅胶GF254薄层板分离葛根素,采用薄层扫描法,以254 nm为检测波长测定消渴丸中葛根素的含量.结果表明葛根素在2.0-10.0μg范围内呈良好的线性关系,y=1064x＋ 16290,r=0.9949,平均回收率为101.1％,RSD=1.7％（n=5）.该法简便、准确、重现性好,可作为消渴丸质量控制的方法.
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| 关 键 词： | 消渴丸 葛根素 薄层扫描法 |
Determination of the Content of Puerarin in Xiaoke Pill by TLC Scanning |
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| CHENG Geng-Jinsheng,PENG Jin-Nian,ZHANG Fu-Yuan,LI Dan-Dan.Determination of the Content of Puerarin in Xiaoke Pill by TLC Scanning[J].Chinese Journal of Spectroscopy Laboratory,2013(6):2915-2917. |
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| Authors: | CHENG Geng-Jinsheng PENG Jin-Nian ZHANG Fu-Yuan LI Dan-Dan |
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| Institution: | (College of Pharmacy, Gannan Medical University, Ganzhou, Jiangzi 341000, P. R. China; a Forestry and Environmental Institute,Jiangxi Environmental Engineerblg Vocational College,Jiangxi 341000,P. R. China) |
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| Abstract: | A TI.CS method was used to determine the content of puerarin in Xiaoke Pill. The adsorbent was silica GF254, the developer consisted of chloroform-methanol-ethyl acetate-water （3 ： 3： 4 ：1） and the determination wavelength was 254nm. The results revealed that the regression equation of puerarin was y=1064x＋16290 （r= 0. 9949）,which was linear over the range of 2.0- 10. 0gg. The average recovery was 101.1 G, RSD=1.7 G （n = 5）. This method is simple and accurate with good repeatability. It was a suitable method in measuring the content of puerarin in Xiaoke Pill. |
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| Keywords: | Xiaoke Pill Puerarin TLC-scanning |
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