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| 维生素B12与牛血清白蛋白相互作用的荧光法研究 | |
| 引用本文： | 樊艳华,冯锋,陈泽忠,双少敏.维生素B12与牛血清白蛋白相互作用的荧光法研究[J].光谱实验室,2011,28(3):1331-1335. |
| 作者姓名： | 樊艳华 冯锋 陈泽忠 双少敏 |
| 作者单位： | 1. 山西大学化学化工学院,太原市坞城路92号,030006 2. 山西大学化学化工学院,太原市坞城路92号,030006;山西大同大学有机化学研究所,山西省大同大学御东校区有机化学研究所,037009 3. 山西大同大学有机化学研究所,山西省大同大学御东校区有机化学研究所,037009 |
| 摘 要： | 用荧光光谱法和紫外吸收光谱法研究了生理条件下维生素B12与牛血清白蛋白之间的相互作用。结果表明,维生素B12对牛血清白蛋白的荧光有猝灭作用,其猝灭过程属于静态猝灭。通过计算得出维生素B12与牛血清白蛋白的结合常数及结合位点数。根据Foerster非辐射能量转移理论,测得维生素B12与牛血清白蛋白的能量转移效率E=0.1091,维生素B12与牛血清白蛋白的结合位置距离212位的色氨酸残基为6.29nm。同时采用同步荧光光谱法研究了维生素B12对牛血清白蛋白构象的影响。 |
| 关 键 词： | 维生素B12 牛血清白蛋白 荧光光谱法 |
Studies on Interaction Between Bovine Serum Albumin and Vitamin B12by Fluorescence Spectrometry |
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| FAN Yan-Huaa FENG Fenga,b CHEN Ze-Zhongb SHUANG Shao-Min.Studies on Interaction Between Bovine Serum Albumin and Vitamin B12by Fluorescence Spectrometry[J].Chinese Journal of Spectroscopy Laboratory,2011,28(3):1331-1335. | |
| Authors: | FAN Yan-Huaa FENG Fenga b CHEN Ze-Zhongb SHUANG Shao-Min |
| Institution: | aa(College of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,P.R.China)b(Institute of Organic Chemistry,Shanxi Datong University,Datong,Shanxi 037009,P.R.China) |
| Abstract: | The interaction between vitamin B12（VB12） and bovine serum albumin（BSA） was investigated by ultraviolet absorption spectrometry and fluorescence spectrometry.The results showed that the fluorescence of BSA was quenched by VB12,which was the static quenching process.The binding constants（KA） and the corresponding numbers of binding sites（n） were obtained by calculation.Based on the mechanism of the foerster energy transference,the transfer efficiency of energy（E） and distance（r） between VB12 and BSA were 0.1091 and 6.29nm,respectively.The influence of VB12 on conformational changes of BSA was investigated by synchronous spectrometry. |
| Keywords: | Vitamin B12 Bovine Serum Albumin Fluorescence Spectrometry |
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