| 2种卤代荧光素滤纸基质室温磷光特性及其与DNA作用的研究 |
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| 引用本文： | 李建晴,侯晓丽,魏玉霞,董川.2种卤代荧光素滤纸基质室温磷光特性及其与DNA作用的研究[J].光谱实验室,2006,23(2):295-299. |
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| 作者姓名： | 李建晴 侯晓丽 魏玉霞 董川 |
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| 作者单位： | 1. 山西晋中学院化学化工系,山西省晋中市,030600
2. 山西大学化学化工学院,太原市,030006 |
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| 基金项目： | 中国科学院资助项目
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山西省自然科学基金 |
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| 摘 要： | 对四溴荧光素(TBF)、四氯四溴荧光素(TTF)两种卤代荧光素滤纸基质室温磷光光谱(PSRTP)特性及其与DNA的作用进行了研究.结果表明:TBF和TTF的PS-RTP的最大λex/λem为526/652nm和557/699nm;酸度实验表明:两种物质在碱性范围内有强发射.小牛胸腺(DNA)的存在会使TBF和TTF的PS-RTP强度发生变化;偏振实验表明:TBF、TTF与DNA的作用方式有嵌插作用;TBF和TTF的磷光寿命分别为136.4ms和131.0ms属长寿命磷光.
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| 关 键 词： | 四溴荧光素 四氯四溴荧光素 DNA 滤纸基质室温磷光 |
| 文章编号： | 1004-8138(2006)02-0295-05 |
| 收稿时间： | 2005-11-06 |
| 修稿时间： | 2005-11-20 |
Study on Phosphorescence Characteristics of Two Halogenated Fluoresceins in Paper Substrate at Room Temperature and Their Interaction with DNA |
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| LI Jian-Qing,HOU Xiao-Li,WEI Yu-Xia,DONG Chuan.Study on Phosphorescence Characteristics of Two Halogenated Fluoresceins in Paper Substrate at Room Temperature and Their Interaction with DNA[J].Chinese Journal of Spectroscopy Laboratory,2006,23(2):295-299. |
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| Authors: | LI Jian-Qing HOU Xiao-Li WEI Yu-Xia DONG Chuan |
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| Institution: | Institute of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,P.R.China;a.Institute of Chemistry and Chemical Engineering,Jinzhong College,Jinzhong,Shanxi 030600,P.R.China |
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| Abstract: | The phosphorescence characteristics of two halogen fluoresceins of tetrabromofluor-escein(TBF) and tetrachlorotetrabromofluorescein(TTF) in paper substrate at room temperature and their interaction with DNA were studied.The maximal excitation and emission wavelengths were 526nm/652nm for TBF and 557nm/699nm for TTF,respectively,and they can emit strong RTP in basic medium with lifetime of 136.4ms and 131.0ms,respectively.The PS-RTP intensities of TBF and TTF are changed in the presence of DNA with TBF and TTF intercalations. |
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| Keywords: | Tetrabromofluorescein Tetrachlorotetrabromofluorescein DNA Paper Substrate Room Temperture Phosphorescence |
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