| 高氯酸萃取-连续流动法测定烟草中的淀粉含量 |
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| 引用本文： | 吴玉萍,邵岩,赵立红,邓建华,王东丹,宋春满.高氯酸萃取-连续流动法测定烟草中的淀粉含量[J].光谱实验室,2006,23(3):488-492. |
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| 作者姓名： | 吴玉萍 邵岩 赵立红 邓建华 王东丹 宋春满 |
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| 作者单位： | 云南省烟草科学研究所,云南省玉溪市聂耳路47号,653100 |
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| 基金项目： | 云南省烟草公司资助项目 |
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| 摘 要： | 淀粉是烟草中较难测定的成分之一.为了快速准确地测定烟草中的淀粉含量,研究了高氯酸萃取-连续流动法测定烟样中淀粉含量的方法,即每0.1g烟草样品用1.0mL 50%的乙醇和1.2mL 72%高氯酸萃取烟草样品中的淀粉,通过自动化学分析仪(滤光片波长660nm)测定淀粉含量.采用本法测定了云南烤烟烟叶、杀青烟叶、白肋烟和香料烟的淀粉含量,平均回收率为96.71%,相对标准偏差在1.79%-5.58%之间.与传统的酸解法和酶解法比较,本法操作步骤简单、检测成本低、快速准确.
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| 关 键 词： | 淀粉 高氯酸萃取 连续流动法 烟草 |
| 文章编号： | 1004-8138(2006)03-0488-05 |
| 收稿时间： | 2005-12-13 |
| 修稿时间： | 2005-12-20 |
Determination of Starch from the HClO4 Extraction of Tobacco by Continuous Flow Method |
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| WU Yu-Ping,SHAO Yan,ZHAO Li-Hong,DENG Jian-Hua,WANG Dong-Dan,SONG Chun-Man.Determination of Starch from the HClO4 Extraction of Tobacco by Continuous Flow Method[J].Chinese Journal of Spectroscopy Laboratory,2006,23(3):488-492. |
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| Authors: | WU Yu-Ping SHAO Yan ZHAO Li-Hong DENG Jian-Hua WANG Dong-Dan SONG Chun-Man |
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| Institution: | Yunnan Tobacco Science Research Institute , Yuaci , Yunnan 653100,P. R. China |
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| Abstract: | Starch in tobacco is difficult to determine. In this paper ,starch was fast and accurately determined by spectrophotometry at 660nm with HClO4 extraction. The average recovery is 96.71% with the relative standard deviation of 1.79 %-5.58%. Compared with the conventional enzymic and acidic hydrolytic methods, this method is simple, cheap, rapid and accurate, and was applied to the determination of starch in burley tobacco, flue-cured tobacco oriental tobacco and de-enzymed tobacco with satisfactory results. |
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| Keywords: | Starch HClO_4 Extraction Continuous Flow Method Tobacco |
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