| GPC-LS联用法研究魔芋葡甘聚糖分子链形态 |
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| 引用本文： | 陈士勇,汪超,吕文平,陈文平,江贵林,姜发堂,钟晓玲.GPC-LS联用法研究魔芋葡甘聚糖分子链形态[J].光谱实验室,2010,27(1):238-242. |
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| 作者姓名： | 陈士勇 汪超 吕文平 陈文平 江贵林 姜发堂 钟晓玲 |
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| 作者单位： | 1. 湖北工业大学生物工程学院武汉市洪山区李家墩一村一号,430068
2. 湖北工业大学生物工程学院武汉市洪山区李家墩一村一号,430068;武汉力诚生物科技有限公司武汉市银光大道2号飞翔科技工业园1栋 430068 |
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| 基金项目： | 武汉市晨光计划(批准号:20065004116-52);;湖北省教育厅(批准号:20063036) |
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| 摘 要： | 采用凝胶渗透色谱-激光光散射联用(GPC-LS)研究了经酶解后的魔芋葡甘聚糖(KGM)重均分子量(Mw)、均方根旋转半径(Rg)、构象指数(β)与温度的相关性,并结合KGM的特性粘度拟合酶解KGM的Mark-Houwink方程。结果显示:Rg与Mw表现出近似的正相关性,且Mw、Rg及β随温度升高而逐渐增大,酶解KGM的Mark-Houwink方程为η]=5.9×10-1Mw0.562。
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| 关 键 词： | 魔芋葡甘聚糖 分子量 激光光散射 均方根旋转半径 特性粘度 分子链形态 |
Study on Molecular Chain Morphology of Konjac Glucomannan with GPC-Light Scattering |
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| CHEN Shi-Yong,WANG Chao,LV Wen-Ping,CHEN Wen-Ping,JIANG Gui-Lin,JIANG Fa-Tang,ZHONG Xiao-Ling.Study on Molecular Chain Morphology of Konjac Glucomannan with GPC-Light Scattering[J].Chinese Journal of Spectroscopy Laboratory,2010,27(1):238-242. |
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| Authors: | CHEN Shi-Yong WANG Chao LV Wen-Ping CHEN Wen-Ping JIANG Gui-Lin JIANG Fa-Tang ZHONG Xiao-Ling |
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| Institution: | Bioengineering College;Hubei University of Technology;Wuhan 430068;P.R.China;Wuhan Licheng Biotechnology Co.;Ltd.;P.R.China |
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| Abstract: | The correlation between the molecular weight(Mw)of konjac glucomannan(KGM)after enzymatic hydrolysis,root mean square ratio of gyration(Rg),conformation index(β)and temperature were studied by gel permeation chromatograph-laser light scattering,and combined with the intrinsic viscosity of KGM which fitted the Mark-Houwink equation.The Rg and Mw exhibit similar positive correlation,and Mw,Rg and β inhence with the temperature.The enzymolysis of KGM follows the Mark-Houwink equation ofη]=5.9×10~(-1)Mw~(0.562). |
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| Keywords: | Konjacglucomannan Molecular Weigh Laser Light Scattering Root Mean Square Ratio of Gyration Intrinsic Viscosity Molecular Chain Morphology |
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