| 共振瑞利散射光谱法测定人体血浆和尿液中的加替沙星 |
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| 引用本文： | 韩权,王媚,田丽,杨娜.共振瑞利散射光谱法测定人体血浆和尿液中的加替沙星[J].光谱实验室,2010,27(6):2146-2150. |
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| 作者姓名： | 韩权 王媚 田丽 杨娜 |
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| 作者单位： | [1]西安文理学院化学与化学工程系,西安市太白南路168号710065 [2]陕西师范大学化学与材料科学学院,西安市710062 |
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| 摘 要： | 提出了共振瑞利散射光谱法测定加替沙星的新方法。在pH5.2—5.6的Britton-Robinson缓冲溶液中,加替沙星(Gatifloxacin,GTFX)与钴(Ⅱ)能形成阳离子配合物,它可进一步与酸性染料刚果红(CR)阴离子反应形成2:1:1(GTFX:Co~(2+):CR)的三元离子缔合物,使共振瑞利散射(RRS)急剧增强,其2个散射峰分别位于382nm和560nm处。在382nm处,加替沙星的浓度在0—5.26μg·mL~(-1)范围内,与RRS强度有良好的线性关系,检出限(3σ)为7.5ng·mL~(-1)。此方法简便、快速,且具有良好的选择性,用于片剂、尿液和血浆中加替沙星的测定,其回收率在96.1%—103.6%。
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| 关 键 词： | 加替沙星 Co2＋ 刚果红 共振瑞利散射 |
Determination of Gatifloxacin in Blood and Urine by Resonance Rayleigh Scattering Method |
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| HAN Quan,WANG Mei,TIAN Li,YANG Na.Determination of Gatifloxacin in Blood and Urine by Resonance Rayleigh Scattering Method[J].Chinese Journal of Spectroscopy Laboratory,2010,27(6):2146-2150. |
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| Authors: | HAN Quan WANG Mei TIAN Li YANG Na |
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| Institution: | (Department of Chemistry,Xi＇an University of Arts and Science,Xi＇an 710065 .P. R. China; School of Chemistry and Materials Science, Shaanxi Normal University, Xi＇an 710062, P. R. China) |
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| Abstract: | A novel method for the determination of gatifloxaein by resonance Rayleigh scattering （RRS） has been developed. In pH 5.2-5.6 Britton-Robinson buffer solution,gatifloxacin can react with Co2＋ to form cation chelates, which further bound with Congo Red to form ternary ion-association complexes（GTFX ： Co2＋ ： CR=2 ： 1 ： 1）,resulting in a significant enhancement of resonance Rayleigh scattering（RRS）. The two scattering peaks are located at 382nm and 560nm. The RRS intensity is proportional to the concentration of gatifloxacin in the range of 0-5.26ug ·mL-1 and its detection limit is 7. 5 ng· mL-1. The method, which is simple, rapid and have a good selectivity,has been successfully applied to the determination of gatifloxacin in tablet,human urine and serum and its recovery ranges from 96.1%--103.6%. |
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| Keywords: | Gatifloxacin Co2＋ Congo Red Resonance Rayleigh Scattering |
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