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高效液相色谱-紫外检测法测定食品中的过氧化氢
引用本文:陈易晖,刘艳,周建立,朱海维,项丽霞.高效液相色谱-紫外检测法测定食品中的过氧化氢[J].光谱实验室,2009,26(2):414-417.
作者姓名:陈易晖  刘艳  周建立  朱海维  项丽霞
作者单位:象山出入境检验检疫局,浙江省象山县兴盛路228号,315700
摘    要:通过在60℃下超声提取,用亚铁氰化钾和乙酸锌沉淀蛋白质,通过0.45μm滤膜净化样品。利用水和乙腈在200nm波长紫外吸收弱的特点,降低基线噪音。在0.5mL/min流速下,色谱柱对过氧化氢的保留增大,可以与杂质较好分离。用反相高效液相色谱-紫外检测色谱法,在C18柱上不经过衍生化测定了食品中过氧化氢的残留。过氧化氢的检出限为1.5×10^-7mol/L。对实际样品进行检测时,平均回收率R≥75%,RSD≤3%。

关 键 词:高效液相色谱  食品  过氧化氢

Determination of Peroxides in Food Samples by High Performance Liquid Chromatography with Variable Wavelength Detector
CHEN Yi-hui,LIU Yan,ZHOU Jian-Li,ZHU Hai-Wei,XIANG Li-Xia.Determination of Peroxides in Food Samples by High Performance Liquid Chromatography with Variable Wavelength Detector[J].Chinese Journal of Spectroscopy Laboratory,2009,26(2):414-417.
Authors:CHEN Yi-hui  LIU Yan  ZHOU Jian-Li  ZHU Hai-Wei  XIANG Li-Xia
Institution:Xiangshan Entry-Exit Inspection and Quarantine;Xiangshan;Zhejiang 315700;P.R.China
Abstract:Hydrogen peroxide(H2O2) was determined without derivatization by reversed phase-high performance liquid chromatographic(RP-HPLC).A Zorbax ODS column (250 mm×4.6mm i.d.,5μm) was used with acetontrile-water(10∶90,V/V) as mobile phase at flow rate of 0.5mL/min,and detected at 200nm.The baseline noise was reduced because acetontrile-water had much lower absorption at 200nm,and the sensitivity was thus improved.The limit of detection of H2O2 was 1.5×10^-7mol/L.The average recovery is more than 75% and relative standard deviation is less than 3%.
Keywords:High Performance Liquid Chromatography  Food Samples  Hydrogen Peroxide    
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