| 火棘果中总黄酮的含量测定 |
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| 引用本文： | 甘秀海,陈华国,周欣,赵超.火棘果中总黄酮的含量测定[J].光谱实验室,2012,29(2):1223-1226. |
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| 作者姓名： | 甘秀海 陈华国 周欣 赵超 |
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| 作者单位： | 1. 贵州师范学院化学与生命科学学院,贵阳市乌当区高新路115号,550018
2. 贵州师范大学天然药物质量控制研究中心,贵阳市宝山北路116号,550001 |
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| 基金项目： | 贵州省科技创新人才团队建设项目[黔科合人才团队(2011)4008];贵阳市科学技术计划项目([2010]筑科计合同字第3-重-1号) |
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| 摘 要： | 利用索氏提取法提取火棘果中总黄酮,以芦丁为对照品,紫外分光光度法测定其含量。在波长418nm处芦丁在浓度5—30μg.mL-1范围内具有良好线性关系,其相关系数r=0.9997。该方法简便、重现性好,可作为火棘中总黄酮含量测定的一种方法。
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| 关 键 词： | 火棘果 芦丁 总黄酮 紫外分光光度法 |
Determination of Total Flavonoids in Fruit of Pyracantha fortuneana(Maxim.) Li |
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| GAN Xiu-Hai
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CHEN Hua-Guo
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ZHOU Xin
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ZHAO Chao.Determination of Total Flavonoids in Fruit of Pyracantha fortuneana(Maxim.) Li[J].Chinese Journal of Spectroscopy Laboratory,2012,29(2):1223-1226. |
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| Authors: | GAN Xiu-Hai CHEN Hua-Guo ZHOU Xin ZHAO Chao |
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| Institution: | GAN Xiu-Hai CHEN Hua-Guoa ZHOU Xina ZHAO Chaoa(The School of Chemistry and Life Science,Guizhou Normal College,Guiyang 550018,P.R.China)a(The Research Center for Quality Control of Natural Medicine,Guizhou Normal University,Guiyang 550001,P.R.China) |
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| Abstract: | The total flavonoids in the fruit of Pyracantha fortuneana(Maxim.) Li were extracted by Soxhlet extraction method and determined by UV spectrophotometry with rutin as reference standard.The rutin had a good linear relationship in the concentration range of 5—30μg·mL-1,and the correlation coefficient was 0.9997 at 418nm.The method is simple and accurate with good reproducibility,and can be used to determine the content of total flavones in Pyracantha fortuneana(Maxim.)Li. |
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| Keywords: | Fruit of Pyracantha fortuneana(Maxim )Li Rutin Total Flavonoids UV Spectrophotometry |
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