| 7种常见食用菌中多糖的提取和测定 |
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| 引用本文： | 李承范.7种常见食用菌中多糖的提取和测定[J].光谱实验室,2012,29(2):996-999. |
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| 作者姓名： | 李承范 |
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| 作者单位： | 延边大学分析测试中心,吉林省延吉市公园路977号,133002 |
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| 基金项目： | 吉林省教育厅科技计划项目:吉教科合字[2009]14号 |
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| 摘 要： | 采用热水提取、乙醇沉淀的方法从7种食用菌中提取出了水溶性多糖,采用蒽酮-硫酸法在波长620nm处进行了糖含量的测定,求得其校准曲线的回归方程为y=0.0094x+0.0057,相关系数r为0.9998。平均回收率为98.3%—102.7%,RSD为2.1%—3.2%。结果显示粗多糖的提取率为4.44%—16.75%,多糖含量为3.48%—6.91%。
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| 关 键 词： | 食用菌 水溶性多糖 提取 蒽酮-硫酸法 |
Extraction and Determination of Polysaccharides from Seven Kind of Common Edible Fungus |
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| LI Cheng-Fan.Extraction and Determination of Polysaccharides from Seven Kind of Common Edible Fungus[J].Chinese Journal of Spectroscopy Laboratory,2012,29(2):996-999. |
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| Authors: | LI Cheng-Fan |
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| Institution: | LI Cheng-Fan(Analysis and Inspection Center of Yanbian University,Yanji,Jilin 133002,P.R.China) |
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| Abstract: | Hydrosoluble polysaccharides were extracted from seven kinds of common edible fungus by hot water-ethanol precipitation method.The contents of polysaccharides were determined by anthrone-sulfuric acid method at wavelength of 620nm,thus the regression equation of calibration curve was y=0.0094x+0.0057 with correlation coefficient of 0.9998.The average recoveries were 98.3%—102.7% with relative standard deviations(RSDs) of 2.1%—3.2%.The extraction efficiencies of polysaccharides were 4.44%—16.75%,and the contents of polysaccharides were 3.48%—6.91%. |
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| Keywords: | Edible Fungus Hydrosoluble Polysaccharides Extraction Anthrone-Sulfuric Acid Method |
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