| 荷移分光光度法测定缬氨酸 |
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| 引用本文： | 韩玲军,李省云.荷移分光光度法测定缬氨酸[J].光谱实验室,2012,29(1):503-505. |
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| 作者姓名： | 韩玲军 李省云 |
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| 作者单位： | 太原师范学院(南区)化学系 030031 |
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| 基金项目： | 国家自然科学基金，山西省高校科技开发项目 |
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| 摘 要： | 用分光光度法研究缬氨酸与7,7,8,8-四氰基对苯醌二甲叉(TCNQ)的荷移反应.确定以pH=9.2的三酸缓冲溶液为介质,在50C的水浴中加热30min,形成稳定的络合物,其λmax=417nm,表观摩尔吸光系数ε=7.8×103L·mol-1·cm-1,在1-9μg/mL的范围内符合比耳定律,回收率在96.7％-102.7％,相对标准偏差小于2.9％.
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| 关 键 词： | 分光光度法 荷移反应 缬氨酸 7 7 8 8-四氰基对苯醌二甲叉 |
Determination of Valine by Charge-Transfer Spectrophotometry |
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| HAN Ling-Jun
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LI Sheng-Yun.Determination of Valine by Charge-Transfer Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2012,29(1):503-505. |
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| Authors: | HAN Ling-Jun LI Sheng-Yun |
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| Institution: | (Department of Chemistry,Taiyuan Normal University,Taiyuan 030031,P.R.China) |
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| Abstract: | The charge-transfer reaction of valine with 7,7,8,8-tetracyanoquinodimethane （TCNQ） was investigated by spectrophotometry.The experimental conditions were optimized and a new method for determination of valine has been developed.The stable charge-transfer complex was formed in Britton-Robinson buffer solution（pH=9.2） by heating in 50’C water bath for 30min.The apparent molar absorptivity of the complexεwas determined for 7.8×103L · mol-1 · cm-1 at 417nm.Beer’s Law was obeyed in the range of 1—9μg/mL of valine.The recovery was 96.7%—102.7%,and the relative standard deviation was less than 2.9%. |
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| Keywords: | Spectrophotometry Charge-Transfer Reaction Valine TCNQ |
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