固相萃取-高效液相色谱测定污水中的地塞米松

建立固相萃取-高效液相色谱法测定污水中地塞米松含量的方法。水样经固相萃取柱萃取富集后进行HPLC分析,以甲醇:水=65:35(V/V)为流动相,色谱柱为Agilent C18柱(5μm,4.6mm×150mm),柱温30℃;流速为1mL/min;紫外检测波长为240nm。地塞米松在25—300ng/mL范围内线性关系良好(n=5,R2=0.9992),最低检测浓度为25ng/mL(S/N>10),回收率为98.36%,地塞米松的低、中、高的日内精密度与日间精密度RSD均小于3%。本法准确、灵敏,能较好的应用于污水中地塞米松的测定。

Determination of Dexamethasone in Sewage by SPE-HPLC

The method for the determination of the content of dexamethasone in sewage by solid phase extraction-high performance liquid chromatography(SPE-HPLC) was established.The water samples were enriched by solid phase extraction column,then determined by HPLC on a Agilent C18(5μm,4.6mm×150mm) chromatographic column with mobile phase consisting of a mixture of methanol and water(65∶35,V/V) at a flow rate of 1mL/min.The ultraviolet detection wavelength was 240nm,and the column temperature was 30℃.There was the good linear relation for the concentration of dexamethasone in the range of 25—300ng/mL(n=5,R2=0.9992),and the limit of quantitation(LOQ,S/N>10) was found to be 25ng/mL(S/N>10).The recovery was 98.36%.The relative standard deviation(RSD) of the intra-day and the inter-day were both less than 3%.This method is accurate and sensitive,that can be applied to determining dexamethasone in sewage.