光谱法研究环糊精与艾地苯醌的超分子作用

用荧光光谱法研究了药物艾地苯醌与β-环糊精在溶液中的超分子复合作用,发现在加入β-环糊精后其荧光强度显著增强;Benesi-Hildebrand曲线显示在溶液中它们之间以1∶1的方式结合,包合常数K为412mol/L;紫外滴定实验显示出艾地苯醌的去质子化常数在加入β-CD后与本身的pKa基本一致,说明超分子复合物中艾地苯醌分子中的—OH基团存在于环糊精空腔外面。核磁测试显示在加入β-CD后艾地苯醌的C-11的化学位移向高场移动,而C-11上的氢位移则向低场移动,表明芳环上的甲氧基进入了环糊精的空腔。

Spectroscopic Characterization of Supramolecular Complexation of Idebenone with Cyclodextrin

The supramolecular complex formed between β-cyclodextrin （CD） and the drug idebenone in aqueous solution was charactered through the fluorescence spectra. ,addition of β-CD,the fluorescence emission signals of idebenone was obviously intensified,β-CD formed a 1 ： 1 inclusion complex with idebenone. The equilibrium constant was calculated as 412mol/L. Deprotonation constants of idebenone in the presence of β-CD from the absorptimetric titration profile were compared well with that without β-CD, it suggested that the OH group stayed outside the CD cavity in the supramolecular complex. NMR spectra showed that the idebenone＇carbons C-11 undergoes a upfield shift,and the H atom in C-11 undergoes downfield shift after addition of β-CD. The methyoxy group at the aromatic ring is inserted deeply into the β-CD molecule.