| 花生中黄酮的荧光分光光度法测定 |
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| 引用本文： | 陈玉锋,庄志萍,曹静.花生中黄酮的荧光分光光度法测定[J].光谱实验室,2011,28(3):1021-1025. |
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| 作者姓名： | 陈玉锋 庄志萍 曹静 |
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| 作者单位： | 牡丹江师范学院化学化工学院,黑龙江省牡丹江市爱民区文化街19号,157012 |
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| 基金项目： | 牡丹江师范学院重点项目(Z2008003);牡丹江师范学院指导项目(KZ2008008) |
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| 摘 要： | 研究了花生中黄酮的荧光分光光度法测定条件,用芦丁为标准样品,在荧光激发波长λex=265nm,发射波长λem=331nm处测定了花生中黄酮的含量,回归方程为y=5.0775x＋46.716,相关系数r=0.9994,精密度（RSD）为0.28%（n=5）,加标回收率为93.75%—115.00%,该方法用于测定花生中黄酮的含量结果准确。
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| 关 键 词： | 花生 黄酮 荧光分光光度法 |
Determination of Flavones in Peanut by Fluorescence Spectrophotometry |
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| CHEN Yu-Feng ZHUANG Zhi-Ping CAO Jing.Determination of Flavones in Peanut by Fluorescence Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2011,28(3):1021-1025. |
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| Authors: | CHEN Yu-Feng ZHUANG Zhi-Ping CAO Jing |
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| Institution: | CHEN Yu-Feng ZHUANG Zhi-Ping CAO Jing(College of Chemistry and Chemical Engineering,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012,P.R.China) |
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| Abstract: | The conditions of determination for flavones in peanut were studied.The rutin was used as standard sample,and fluorescence excitation wavelength and emission wavelength were set at 265nm and 331nm for determination of flavones by fluorescence spectrophotometry,respectively.The regression equation was y=5.0775x＋46.716,and the correlation coefficient was 0.9994,while the precision was 0.28%（n=5）,and the recovery was 93.75%—115.00%.This method is fitted for determining the content of the flavones in peanut with satisfactory result. |
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| Keywords: | Peanut Flavones Fluorescence Spectrophotometry |
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