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| 珙桐叶总黄酮提取工艺及体外抗氧化性 | |
| 引用本文： | 张悦,苏智先,罗明华,胡进耀,杨林,钟小翠.珙桐叶总黄酮提取工艺及体外抗氧化性[J].光谱实验室,2011,28(3):987-991. |
| 作者姓名： | 张悦 苏智先 罗明华 胡进耀 杨林 钟小翠 |
| 作者单位： | 1. 西华师范大学生命科学学院,四川省南充市师大路1号,637009;四川省生态与环境保护技术重点实验室,四川省绵阳市仙人路一段30号,621000;四川省遂宁市环境监测中心站,四川省遂宁市明月花园小区桂园,629006 2. 四川省生态与环境保护技术重点实验室,四川省绵阳市仙人路一段30号,621000 3. 绵竹市教师培训中心,四川省绵竹市,618200 |
| 基金项目： | 国家自然科学基金资助项目(30670209); 四川省科技厅应用基础项目(05JY029-107) |
| 摘 要： | 运用正交试验设计探讨了绵竹白云山珙桐叶黄酮的微波辅助提取工艺条件,并对提取液的抗氧化性能进行了研究。结果表明,珙桐叶总黄酮提取的最佳工艺参数：微波功率700W,料液比（g/mL）1∶40,乙醇质量分数浓度为70%,提取时间为2min,测得每克干叶中总黄酮含量为71.03mg。对提取液的抗氧化性研究表明,珙桐叶总黄酮对超氧阴离子自由基、羟基自由基的清除率较好,在实验所选浓度范围内,总还原能力随浓度增加而增强。 |
| 关 键 词： | 珙桐叶 提取工艺 微波 总黄酮 抗氧化 |
Extraction Technology and Antioxidant Activity of Flavonoids from Davidia Involucrata Baill |
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| ZHANG Yuea,b,c SU Zhi-Xianb LUO Ming-Huab HU Jin-Yaob YANG Lind ZHONG Xiao-Cuibabcd.Extraction Technology and Antioxidant Activity of Flavonoids from Davidia Involucrata Baill[J].Chinese Journal of Spectroscopy Laboratory,2011,28(3):987-991. | |
| Authors: | ZHANG Yuea b c SU Zhi-Xianb LUO Ming-Huab HU Jin-Yaob YANG Lind ZHONG Xiao-Cuibabcd |
| Institution: | ZHANG Yuea,b,c SU Zhi-Xianb LUO Ming-Huab HU Jin-Yaob YANG Lind ZHONG Xiao-Cuiba(College of Life Science,China West Normal University,Nanchong,Sichuan 637009,P.R.China)b(Sichuan Provincial Key Laboratory of Ecology and Environmental Technology,Mianyang,Sichuan 621000,P.R.China)c(Sichuan Provincial Center Suining of Environmental Monitoring,Suining,Sichuan 629006,P.R.China)d(Training Center of Teachers in Mianzhu,Mianzhu,Sichuan 618200,P.R.China) |
| Abstract: | The extraction conditions of flavonoids from Davidia involucrata Baill were investigated by orthogonal tests with assistant microwave process,and its antioxidant activity was analyzed as well.The results showed that the optimal technological parameters of flavonoids were as follows：microwave power of 700W,mass/volume ratio of 1∶40,ethanol concentration of 70%,extraction time of 2min.The highest concentration of total flavonoids was 71.03mg per gram dry leaves.The results of antioxidant activity for extraction solution showed that Davidia involucrata Baill had better clearance for superoxide anion and hydroxyl free radicals,and the reduction ability increased with the concentration in experimental selecting concertration range. |
| Keywords: | Davidia Involucrata Baill Extraction Technology Microwave Flavonoid Antioxidant |
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