| UPLC测定烟叶中的氨基酸含量 |
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| 引用本文： | 王文辉,杨俊,王齐,孙晓东,朱艳琴,冯雯.UPLC测定烟叶中的氨基酸含量[J].光谱实验室,2010,27(5):1920-1924. |
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| 作者姓名： | 王文辉 杨俊 王齐 孙晓东 朱艳琴 冯雯 |
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| 作者单位： | 昆明理工大学分析测试研究中心,昆明市学府路253号,650093 |
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| 摘 要： | 采用UPLC法测定烟叶中氨基酸的含量。Waters Acquity UPLC BEH C18色谱柱(2.1mm×100mm,1.7μm),AccQ.Tag Ultraa Eluent A及AccQ.Tag Ultraa Eluent B梯度洗脱,流速为0.7mL·min-1,波长:248nm,柱温55℃。18种氨基酸在0.0030-0.204mg/mL范围内有良好的线性关系(r0.9994),平均回收率(n=6)为98.7%。该方法10min内18种氨基酸可完全分离且具有快速、准确、重复性好的特点。
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| 关 键 词： | 超高效液相色谱 氨基酸 烟叶 |
Determination of Amino Acids in Tobacco by UPLC |
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| WANG Wen-Hui,YANG Jun,WANG Qi,SUN Xiao-Dong,ZHU Yan-Qing,FENG Wen.Determination of Amino Acids in Tobacco by UPLC[J].Chinese Journal of Spectroscopy Laboratory,2010,27(5):1920-1924. |
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| Authors: | WANG Wen-Hui YANG Jun WANG Qi SUN Xiao-Dong ZHU Yan-Qing FENG Wen |
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| Institution: | (Research Center for Analysis and Measurement,Kunming University of Science and Technology,Kunming 650093,P.R.China) |
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| Abstract: | A method for determination of the amounts of amino acids in tobacco by UPLC was established.A Waters Acquity UPLC BEH C18 column（2.1mm×100mm,1.7μm）was applied,using AccQ.Tag Ultraa Eluent A and AccQ.Tag Ultraa Eluent B as mobile phase,with a flow rate of 0.7mL·min-1,and the wavelength was 248nm,the column temperature was 55℃.The calibration curve was linear in the range of 0.0030—0.204mg/mL of amino acids（r0.9994）.The average recovery of amino acids was 98.7%.This method has the advantages of rapidity and accurate,repeatability in determination of amino acids in tobacco in ten minutes. |
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| Keywords: | UPLC Amino Acids Tobacco |
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