| 肌红蛋白与生物碱相互作用的光谱性质研究 |
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| 引用本文： | 杨媛,林琳,张伟,李永红.肌红蛋白与生物碱相互作用的光谱性质研究[J].光谱实验室,2009,26(1):122-125. |
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| 作者姓名： | 杨媛 林琳 张伟 李永红 |
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| 作者单位： | 郑州大学化学系分析化学专业,郑州市科学大道100号,450001 |
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| 基金项目： | 河南省基础与前沿技术研究计划 |
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| 摘 要： | 用分子荧光光谱法和紫外可见分光光度法研究了肌红蛋白（myoglobin,Mb）与咖啡因、茶碱相互作用的光谱性质变化。据Stern-Volmer图得到猝灭常数,由紫外-可见吸收光谱判断均为静态猝灭,通过热力学常数得出其相互作用力为静电作用,依据Foerster偶极-偶极无辐射能量转移理论计算出Mb与生物碱间的距离,并用同步荧光光谱技术考察了生物碱对Mb构象的影响。
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| 关 键 词： | 肌红蛋白 生物碱 分子荧光光谱法 |
Study on the Interaction between Myoglobin and Alkaloid by Fluorescence Spectroscopy |
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| YANG Yuan,LIN Lin,ZHANG Wei,LI Yong-Hong.Study on the Interaction between Myoglobin and Alkaloid by Fluorescence Spectroscopy[J].Chinese Journal of Spectroscopy Laboratory,2009,26(1):122-125. |
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| Authors: | YANG Yuan LIN Lin ZHANG Wei LI Yong-Hong |
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| Institution: | Department of Chemistry;Zhengzhou University;Zhenzhou 450001;P.R.China |
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| Abstract: | The interaction of Mb and alkaloid(caffeine,theophylline) was studied by fluorescence spectrometry and ultraviolet spectrophotometry. The quenching mechanism of Mb with alkaloid was suggested as static quenching in teams of Stern-Volmer curves,conjugation effort was mainly electrostatic interaction according to the calculation of thermodynamic parameters,and the distance between them was estimated smaller than 7nm by energy transfer theory. The binding feature of alkaloid to Mb was studied by the synchronou... |
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| Keywords: | Myoglobin Alkaloid Fluorescence Spectrometry |
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