高效液相梯度洗脱法测定银黄含片中黄芩苷、绿原酸和芦丁

建立了反相高效液相色谱法同时测定银黄含片中的黄芩苷、绿原酸和芦丁。采用HypersilODSC18柱(200mm×4.6mm,粒径5μm),流动相A:甲醇-水-乙酸(10∶88∶2)和流动相B:甲醇-水-乙酸(88∶10∶2),梯度洗脱程序0min(A∶B/97∶3)-10min(A∶B/73∶27)-25min(A∶B/20∶80)-36min(A∶B/20∶80);流速1.0mL/min;检测波长为327nm和280nm;柱温25℃。黄芩苷线性范围为0.051—0.512μg/mL,r=0.9990,样品的平均加标回收率为98.23%,(RSD为2.13%);绿原酸线性范围为0.059—0.592μg/mL,r=0.9996,样品的平均加标回收率为97.61%,(RSD为2.13%);芦丁线性范围为0.087—0.868μg/mL,r=0.999,样品的平均加标回收率为98.56%,(RSD为1.13%),该方法专属性强,结果稳定,重现性好,能有效地控制药品的主成分含量,具有较强的实用价值。

Determination of Baicalin, Chlorogenic Acid and Rutin in the Yin Huang Buccal Tablets by HPLC with Gradient Elution

A method for simultaneous determination of baicalin,chlorogenic acid and rutin in the yin huang buccal tablets was developed.A hypersil ODS C18（4.6mm×200mm,5μm） column was used.A mixture of methanol-water-acetic acid（10：88：2）was used as mobile phase A and the mixture of methanol-water-acetic acid（88：10：2） as mobile phase B.Phase A-phase B in gradient elution：0 min （97：3）-10min（73：27）-25min（20：80）-36min（20：80） at a flow rate of 1.0mL/min.The detection wavelength was set at 327 nm and 280 nm and the column temperature was 25℃.The calibration curve was linear in the range of 0.051—0.512μg/mL for baicalin（r=0.9990）,average recovery was 98.23% and RSD was 2.13% （n=6）.The calibration curve was linear in a range of 0.059—0.592μg/mL for chlorogenic acid（r=0.9996）,average recovery was 97.61% and RSD was 2.13%（n=6）.The calibration curve was linear in a range of 0.087—0.868μg/mL for rutin（r=0.9992）,average recovery was 98.56% and RSD was 1.13%（n=6）.The method is sensitive,reproducible,and suitable for the quality control of the yin huang buccal tablets.