| 核黄素与鲱鱼精DNA相互作用的荧光光谱 |
| |
| 引用本文： | 付彩霞,张怀斌,李光.核黄素与鲱鱼精DNA相互作用的荧光光谱[J].光谱实验室,2011,28(4):1727-1730. |
| |
| 作者姓名： | 付彩霞 张怀斌 李光 |
| |
| 作者单位： | 滨州医学院化学教研室 山东省烟台市莱山区观海路346号264003 |
| |
| 摘 要： | 荧光光谱法研究了核黄素(RF)与鲱鱼精DNA的相互作用.考查了离子强度、温度及磷酸盐对RF-DNA体系的影响.结果表明,DNA的存在使得RF的荧光光谱发生了规律性猝灭,其猝灭机制为静态猝灭；运用Stern-Volmer方程对实验数据进行了分析,得到了猝灭常数为2.82×103L·mol-1、结合位点数为1.17.核黄素...
|
| 关 键 词： | 荧光光谱法 核黄素 鲱鱼精DNA 相互作用 |
Interaction Between Riboflavin and Herring Sperm DNA by Fluorescent Spectrum |
| |
| FU Cai-Xia,ZHANG Huai-Bin,LI Guang.Interaction Between Riboflavin and Herring Sperm DNA by Fluorescent Spectrum[J].Chinese Journal of Spectroscopy Laboratory,2011,28(4):1727-1730. |
| |
| Authors: | FU Cai-Xia ZHANG Huai-Bin LI Guang |
| |
| Institution: | FU Cai-Xia ZHANG Huai-Bin LI Guang(Teaching and Research Section of Chemistry,Binzhou Medical College,Yantai,Shandong 264003,P.R.China) |
| |
| Abstract: | The interaction between the riboflavin（RF） and herring sperm DNA was investigated by fluorescent spectrometry.The influences of ionic strength,temperature and phosphate on the RF-DNA system were studied.The fluorescence spectrum of the RF was quenched by DNA,and the mechanism of action was a static quenching process.The quenching data were analyzed by Stern-Volmer equation,and the quenching constant of 2.82×103L·mol-1 and binding site of 1.17 were obtained.The thermal denaturation temperature of DNA in the presence of the RF indicated the mode of action between RF and DNA was not a classical intercalation mode.The salt effect showed that the interaction of the RF with DNA was mainly electrostatic interaction. |
| |
| Keywords: | Fluorescent Spectrometry Riboflavin Herring Sperm DNA Interaction |
| 本文献已被 维普 万方数据 等数据库收录！ |
