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穿心莲中总黄酮含量的测定
引用本文:毕和平,韩长日,廖家旺,何猛雄.穿心莲中总黄酮含量的测定[J].光谱实验室,2006,23(2):356-359.
作者姓名:毕和平  韩长日  廖家旺  何猛雄
作者单位:海南师范大学化学系,海口市龙昆南路99号,571158
摘    要:以芦丁为对照品,NaNO2-Al(NO3)3-NaOH体系为显色剂,利用分光光度法测定了穿心莲中的总黄酮含量.确定了总黄酮提取方法、显色剂浓度和显色时间等.回归方程为A=0.00652 11.0329C,相关系数r=0.9997.平均回收率为104.20%,RSD=1.85%(n=5).本法操作简便,可行,重现性好.

关 键 词:总黄酮  分光光度法  穿心莲
文章编号:1004-8138(2006)02-0356-04
收稿时间:2005-11-13
修稿时间:2005-12-05

Determination of Total Flavonoids in Andrographis Paniculata
BI He-Ping,HAN Chang-Ri,LIAO Jia-Wang,HE Meng-Xiong.Determination of Total Flavonoids in Andrographis Paniculata[J].Chinese Journal of Spectroscopy Laboratory,2006,23(2):356-359.
Authors:BI He-Ping  HAN Chang-Ri  LIAO Jia-Wang  HE Meng-Xiong
Institution:Department of Chemistry, Hainan Normal University, Haikou, Hainan 571158, P. R. China
Abstract:The total flavonoids in Andrographis Paniculata were determined by spectrophotometry with rutin standard and NaNO_2-Al(NO_3)_3-NaOH system.The regression equation is A=0.00652 11.0329C,r=0.9997.The average recovery was 104.20% with the RSD of 1.85%(n=5).The method is simple,reproducible and suitable for the determination of total flavones.
Keywords:Total Flavonoids  Spectrophotometry  Andrographis Paniculata
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