DAN荧光法等3种测定蛹虫草菌丝体中硒含量的方法比较

通过DAN荧光法,3,3-二氨基联苯胺分光光度法和邻苯二胺紫外分光光度法测定蛹虫草菌丝体中硒的含量,对测得结果进行统计分析,并对其检出限、回收率进行比较研究.研究结果表明,DAN荧光法、3,3-二氨基联苯胺分光光度法和邻苯二胺紫外分光光度法中,硒含量相对标准偏差分别是2.5%,9.2%和6.1%;线性回归系数都大于0....

Comparison of DAN Fluorescence and Two Other Analytical Methods for Determination of Selenium Content in Cordyceps Militaris

The DAN fluorescence method,3,3-diaminobenzidine spectrophotometry and ultraviole spectrophotometry for the determination of selenium in Cordyceps militaris were comparatively studied.The relative standard deviations（RSDs）of selenium content were 2.5%,9.2% and 6.1%,respectively,linear regression coefficients were higher than 0.9990,and selenium content of 3,3-diaminobenzidine method was the highest.The lowest detection limits（LOD） of three methods were 0.0008,0.1620,0.01117μg/mL,respectively.The recoveries of the three methods were 92.0%—96.0%.Overall,taken the DAN fluorescence method as a reference,ultraviole spectrophotometry is suitable for determination of trace selenium of Cordyceps militaris,3,3-diaminobenzidine spectrophotometry is more suitable for the determination of larger selenium content of sample.