| 刚果红分光光度法测定盐酸环丙沙星 |
| |
| 引用本文： | 潘自红,陈丽华,段晓辉.刚果红分光光度法测定盐酸环丙沙星[J].光谱实验室,2011,28(2):732-734. |
| |
| 作者姓名： | 潘自红 陈丽华 段晓辉 |
| |
| 作者单位： | 平顶山学院化学化工学院,河南省平顶山市建设路西段240号,467000 |
| |
| 基金项目： | 平顶山学院高层次人才科研启动经费资助项目 |
| |
| 摘 要： | 在pH 4.39的乙酸钠-盐酸缓冲溶液中,盐酸环丙沙星与刚果红发生离子缔合反应,其最大吸收波长位于500nm处,表观摩尔吸光系数ε=9.46×104L/（mol.cm）,盐酸环丙沙星浓度在0.8—3.2mg/L范围内呈良好的线性关系,线性回归方程为A=0.3635＋0.01113C（mg/L）,相关系数r=0.9966;检出限为9ng/L;RSD为1.6%,据此建立了测定盐酸环丙沙星的新方法。优化了对盐酸环丙沙星的测定条件。本法仪器设备简单,操作简便,体系稳定,回收率测定结果较满意。
|
| 关 键 词： | 分光光度法 盐酸环丙沙星 刚果红 |
Determination of Ciprofloxacin Hydrochloride by Spectrophotometry with Congo Red |
| |
| PAN Zi-Hong,CHEN Li-Hua,DUAN Xiao-Hui.Determination of Ciprofloxacin Hydrochloride by Spectrophotometry with Congo Red[J].Chinese Journal of Spectroscopy Laboratory,2011,28(2):732-734. |
| |
| Authors: | PAN Zi-Hong CHEN Li-Hua DUAN Xiao-Hui |
| |
| Institution: | PAN Zi-Hong CHEN Li-Hua DUAN Xiao-Hui(College of Chemistry and Chemical Engineering,Pingdingshan College,Pingdingshan,Henan 467000,P.R.China) |
| |
| Abstract: | A simple and rapid spectrophotometric method for the determination of ciprofloxacin hydrochloride was developed based on the interaction between congo red and ciprofloxacin hydrochloride in the buffer solution of NaAc-HCl for pH of 4.39.Under the optimal conditions of determination,there were good linear relationship in the range of 0.8—3.2mg/L for ciprofloxacin hydrochloride at the maximum absorption wavelength of 500nm,with ε of 9.46×104L/（mol·cm）.The equation of linear regression was A=0.3635＋0.01113C（mg/L,r=0.9966）,and the limit of detection was 9ng/L,while RSD was 1.6%.This method is applied to the analysis of ciprofloxacin hydrochloride samples with the simple instrument,convenient operates and the better recovery. |
| |
| Keywords: | Spectrophotometry Ciprofloxacin Hydrochloride Congo Red |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
