| 紫外光分光光度法测定淫羊藿中维生素C的含量 |
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| 引用本文： | 张华峰,张蕾,杨晓华.紫外光分光光度法测定淫羊藿中维生素C的含量[J].光谱实验室,2009,26(6):1508-1512. |
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| 作者姓名： | 张华峰 张蕾 杨晓华 |
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| 作者单位： | 张华峰(陕西师范大学食品工程与营养科学学院西北濒危药材资源开发国家工程实验室,西安市长安南路,710062;中国科学院武汉植物研究所/武汉植物园植物保育遗传学重点实验室,武汉市磨山路,430074);张蕾(中国科学院武汉植物研究所/武汉植物园植物保育遗传学重点实验室,武汉市磨山路,430074);杨晓华(西安交通大学医学院,西安市雁塔西路,710049) |
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| 基金项目： | 国家自然科学基金，陕西师范大学引进高层次人才科学研究项目，陕西师范大学优秀科技预研项目 |
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| 摘 要： | 紫外光分光光度法测定淫羊藿中维生素C含量。测定波长为243nm;维生素C浸提剂为1.0%（V/V）盐酸溶液;维生素C浓度在2—50mg/L范围内与吸光度线性关系良好（r=0.9992,n=3）;回收率在97%—115%之间（n=3）;相对标准偏差都小于6.0%（n=3）;检出限（LOD）和定量限（LOQ）分别为1.33mg/L和4.04mg/L。人工栽培的箭叶淫羊藿、天平山淫羊藿和长蕊淫羊藿鲜叶中维生素C的含量不同。
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| 关 键 词： | 淫羊藿 维生素C 紫外光分光光度法 |
Determination of Vitamin C in Herba Epimedii by Ultraviolet Spectrophotometry |
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| ZHANG Hua-Feng a,b ZHANG Lei b YANG Xiao-Hua c a b c.Determination of Vitamin C in Herba Epimedii by Ultraviolet Spectrophotometry[J].Chinese Journal of Spectroscopy Laboratory,2009,26(6):1508-1512. |
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| Authors: | ZHANG Hua-Feng a b ZHANG Lei b YANG Xiao-Hua c a b c |
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| Institution: | ZHANG Hua-Feng a,b ZHANG Lei b YANG Xiao-Hua c a(National Engineering Laboratory of Endangered Medicinal Materials Resources Development in Northwest China,College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710062,P.R.China) b(Key Laboratory of Plant Conservation Genetics,Wuhan Institute of Botany,Wuhan Botanical Garden,Chinese Acadmy of Sciences,Wuhan 430074,P.R.China) c(School of Medicine,Xi'an Jiaotong University,Xi'an 710049,P.R.China) |
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| Abstract: | Vitamin C in herba epimedii was determined by ultraviolet spectrophoton etry at 243nm; 1.0%(V/V) HCI aqueous solution was selected to extract vitamin C from fresh Epimedium leaves. Calibration curve was found in the range of 2-50mg/L and the correlation coefficient of linear regression analysis was 0.9992 (n=3). Recovery of vitamin C was between 97 % and 115%(n=3). Relative standard deviations(RSDs)were less than 6.0%(n=3). LOD and LOQ were 1.33mg/L and 4.04n,g/L, respectively. A variation of the contents of vitamin C was found among the cultivated samples of E. sagittatum , E. myrianthum and E. dolichostemon. |
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| Keywords: | Herba Epimedii Vitamin C Ultraviolet Spectrophotometry |
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