|
|||||
|
|
| HPLC法同时测定血必净中多指标成分的含量 | |
| 引用本文： | 张韻慧,胡文文,任斯嘉,张崧,娄辉,张旺.HPLC法同时测定血必净中多指标成分的含量[J].光谱实验室,2013,30(5):2305-2308. |
| 作者姓名： | 张韻慧 胡文文 任斯嘉 张崧 娄辉 张旺 |
| 作者单位： | 1. 天津大学药物科学与技术学院 天津市南开区卫津路92号 300072 2. 天津大学化工学院 天津市南开区卫津路92号 300072 3. 天津红日药业股份有限公司 天津市武清开发区泉发路20号 301700 |
| 基金项目： | 天津市自然基金重点项目 |
| 摘 要： | 建立波长切换技术同时测定血必净中芍药苷、羟基红花黄色素A、阿魏酸含量的高效液相分析方法.采用Agilent Zorbax SB-C18柱（4.6mm×250mm,5μm）,以甲醇-0.7％磷酸水溶液为流动相,流速1.0mL·min-1,波长230nm（0-25min）、321nm （25-35min）,柱温30℃.所检测的3种成分线性关系良好,精密度、重复性、稳定性的RSD都低于2％.平均回收率分别为102.6％、100.6％、98.10％.该方法简便、准确、重现性好. |
| 关 键 词： | 血必净 芍药苷 羟基红花黄色素A 阿魏酸 高效液相色谱 |
Simultaneous Determination of Multicomponent in Xuebijing by HPLC |
|
| ZHANG Yun-Hui , HU Wen-Wen , REN Si-Jia , LOU Hui , ZHANG Song , ZHANG Wang.Simultaneous Determination of Multicomponent in Xuebijing by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2013,30(5):2305-2308. | |
| Authors: | ZHANG Yun-Hui HU Wen-Wen REN Si-Jia LOU Hui ZHANG Song ZHANG Wang |
| Institution: | (School of Pharmaceuticals and Biotechnology Tianjin University, Tianjin 300072, P. R. China) a (School of Chemical Engineerblg and Technology Tianjin University,Tianjin 300072,P. R. China) b (Tianjin Chase Sun Pharmaceutical Co. Ltd, Tianjin 301700, P. R. China) |
| Abstract: | The method for the determination of peoniflorin,hydroxysafflor yellow A,and ferulic acid in Xuebijing by HPLC with changing ultraviolet-visible wavelength method was established. The compounds were separated by Agilent Zorbax SB-C18 column （4.6mm）K 250mm, 5btm） with mobile phase of methanol-0. 7 % phosphoric acid at a flow rate of 1.0mL·min-1,the detection wavelength was 230nm（0--25min） and 321nm （25--35min） ,and the column temperature was 30~C. There was a good linear relationship of the three components,the RSD of the accuracy,repeatability,stability were all bellow 2% and the average recovery were 102. 6%, 100. 6% and 98. 1%. This is a simple, scientific,reliable method for the quality control of Xuebijing. |
| Keywords: | Xuebijng Peoniflorin Hydroxysafflor Yellow A Ferulic Acid HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ | |