| 反相高效液相色谱法测定清开灵分散片中栀子苷含量 |
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| 引用本文： | 程齐来,李洪亮,张道英,彭金年.反相高效液相色谱法测定清开灵分散片中栀子苷含量[J].光谱实验室,2010,27(5):2016-2019. |
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| 作者姓名： | 程齐来 李洪亮 张道英 彭金年 |
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| 作者单位： | 1. 赣南医学院药学院,江西省赣州市医学院路1号,341000
2. 赣南医学院药学院,江西省赣州市医学院路1号,341000;江西省高等学校天然药物研究与开发重点实验室,江西省赣州市,341000 |
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| 摘 要： | 对中药复方制剂清开灵分散片中有效成分栀子苷进行含量测定。采用反相高效液相色谱法,Agilent1100ZORBAXEclipseXDB-C18色谱柱(4.6mm×150mm,5μm),乙腈-水(15∶85,V/V)为流动相,检测波长为238nm,流速1.0mL/min,柱温25℃。栀子苷在0.0593—1.4825μg.mL-1范围内与峰面积呈良好的线性关系,r=0.9999,平均加样回收率为98.93%(n=6),RSD=1.21%。该方法快速简便、灵敏度和稳定性高,可有效控制清开灵分散片的内在质量。
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| 关 键 词： | 清开灵分散片 栀子苷 反相高效液相色谱法 |
Determination of Jasminoidin in Qingkailing Dispersible Tablets by RP-HPLC |
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| CHENG Qi-Lai,LI Hong-Liang,ZHANG Dao-Ying,PENG Jin-Nian.Determination of Jasminoidin in Qingkailing Dispersible Tablets by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2010,27(5):2016-2019. |
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| Authors: | CHENG Qi-Lai LI Hong-Liang ZHANG Dao-Ying PENG Jin-Nian |
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| Institution: | CHENG Qi-Laia LI Hong-Lianga ZHANG Dao-Yinga PENG Jin-Niana,ba(Department of Pharmacy,Gannan Medical University,Ganzhou,Jiangxi 341000,P.R.China)b(The Vital Lab of Natural Drugs Research and Development in Jiangxi High Schools,Ganzhou,Jiangxi 341000,P.R.China) |
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| Abstract: | A method for the determination of jasminoidin in qingkailing dispersible tablets（QDT）was established.The RP-HPLC method was performed on Agilent1100 ZORBAX Eclipse XDB-C18 column（4.6mm×150mm,5μm）.Acetonitrile and water were used as the mobile phase（15∶85,V/V）,detection wavelength was at 238nm,the flow rate was 1.0mL/min and the column temperature was at 25℃.In the range of 0.0593—1.4825μg·mL-1 jasminoidin has good linearity with chromatograph peak area（r=0.9999）.The average recovery is 98.93%（n=6）,and RSD is 1.21%.The method is simple,effective and with a higher sensibility and stability,and can be used to control the quality of QDT effectively. |
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| Keywords: | Qingkailing Dispersible Tablets Jasminoidin RP-HPLC |
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