重力对金纳米颗粒在生物膜表面吸附影响的荧光研究

纳米颗粒在生物膜表面的吸附行为是纳米生物技术领域的重要问题. 采用正、倒置实验, 通过荧光显微镜定量研究了重力对金纳米颗粒在支撑磷脂膜表面吸附的影响. 研究发现, 颗粒尺寸决定其在顶或底层支撑膜表面吸附的差异性. 吸附量的差异与颗粒的沉淀速率和扩散速率之比的对数呈线性关系. 粒径小于14 nm时, 不考虑重力在吸附时的影响; 粒径大于176 nm时, 重力在吸附中占主导地位. 为药物载体研究和理解颗粒－生物膜相互作用提供参考.

Effect of gravity on gold nanoparticle adsorption on biomembrane: a fluorescence study

Adsorption of nanoparticles on biomembrane is one critical problem in bionanotechnology. Using fluorescence microscopy, we studied the effect of gravity on gold nanoparticle adsorption on supported lipid membrane by performing the upright and inverted configuration experiments. It is found that: particle size controls the discrepancy between the adsorption on the membranes at the top and bottom of medium. The adsorption disparity increases linearly with the logarithm of the ratio between the sedimentation and diffusion velocities. Gravity is neglectable in adsorption for nanoparticles less than 14 nm, while it is dominant in adsorption for nanoparticles larger than 176 nm. The study provides references for drug carrier research and the understanding of the nanoparticle-membrane interaction in real life.