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| 温度对胶原蛋白结构影响的二维红外相关光谱的研究 | |
| 引用本文： | 肖和兰,孙素琴,周群,庞小峰,蔡国平.温度对胶原蛋白结构影响的二维红外相关光谱的研究[J].原子与分子物理学报,2003,20(2):211-218. |
| 作者姓名： | 肖和兰 孙素琴 周群 庞小峰 蔡国平 |
| 作者单位： | 1. 清华大学生物科学与技术系,生物膜与膜生物工程国家实验室,北京,100084 2. 清华大学化学系分析中心,生命与健康分析化学实验室,北京,100084 3. 成都电子科技大学,生命科学与技术学院,成都,610028 |
| 摘 要： | 采用傅立叶红外光谱法(FTIR)和二维相关分析(2D Correlation Analysis)技术研究了胶原蛋白在加热(15℃～95℃)条件下构象变化的规律.结果表明:二维相关分析能提供由温度变化引起的蛋白构象变化过程中分子结构动态变化的微观信息,为揭示胶原蛋白的结构特征和变温微扰引起的结构变化机理提供了一些有意义的实验依据和初步的理论分析. |
| 关 键 词： | 胶原蛋白 傅立叶变换红外光谱 二维相关分析 |
| 文章编号： | 1000-0364(2003)02-0211-08 |
| 收稿时间： | 2002/10/21 |
| 修稿时间： | 2002年10月21 |
Two-dimensional correlation analysis of FTIR spectrum for collagen during variable temperature denaturing |
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| XIAO He lan ,SUN Su qin ,ZHOU Qun ,PANG Xiao feng ,CAI Guo ping.Two-dimensional correlation analysis of FTIR spectrum for collagen during variable temperature denaturing[J].Journal of Atomic and Molecular Physics,2003,20(2):211-218. | |
| Authors: | XIAO He lan SUN Su qin ZHOU Qun PANG Xiao feng CAI Guo ping |
| Institution: | XIAO He lan 1,SUN Su qin 2,ZHOU Qun 2,PANG Xiao feng 3,CAI Guo ping 1 |
| Abstract: | The Fourier Transform Infrared Spectroscopy and Two Dimensional Correlation Analysis method were applied to study a denaturing process of collagen during varying temperature. It is shown by study results that the microcosmic information of a change in dynamic structure can be provided for collagen during denaturing process. An important theory reference was proposed to produce structural information of collagen and the mechanism of structural variation in the denaturing process |
| Keywords: | Two Dimensional corrleation analysis Fourier transform infrared spectroscopy Collagen |
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