Optical tweezers for confocal microscopy |
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Authors: | A Hoffmann G Meyer zu Hörste G Pilarczyk S Monajembashi V Uhl KO Greulich |
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Institution: | (1) Institut für Molekulare Biotechnologie e.V. (IMB), Abt. Einzelzell- und Einzelmolekültechniken, Beutenbergstrasse 11, 07745 Jena, Germany (Fax: +49-3641/656-410, E-mail: hoan@imb-jena.de), DE |
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Abstract: | In confocal laser scanning microscopes (CLSMs), lasers can be used for image formation as well as tools for the manipulation
of microscopic objects. In the latter case, in addition to the imaging lasers, the light of an extra laser has to be focused
into the object plane of the CLSM, for example as optical tweezers. Imaging as well as trapping by optical tweezers can be
done using the same objective lens. In this case, z-sectioning for 3D imaging shifts the optical tweezers with the focal plane
of the objective along the optical axis, so that a trapped object remains positioned in the focal plane. Consequently, 3D
imaging of trapped objects is impossible without further measures. We present an experimental set-up keeping the axial trapping
position of the optical tweezers at its intended position whilst the focal plane can be axially shifted over a distance of
about 15 μm. It is based on fast-moving correctional optics synchronized with the objective movement. First examples of application
are the 3D imaging of chloroplasts of Elodea densa (Canadian waterweed) in a vigorous cytoplasmic streaming and the displacement
of zymogen granules in pancreatic cancer cells (AR42 J).
Received: 24 March 2000 / Revised version: 23 June 2000 / Published online: 11 October 2000 |
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Keywords: | PACS: 87 64 -t 87 16 -b |
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