Preparation of enzyme nanoparticles and studying the catalytic activity of the immobilized nanoparticles on polyethylene films |
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Authors: | David Meridor Aharon Gedanken |
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Institution: | Department of Chemistry and Kanbar Laboratory for Nanomaterials, Center for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel |
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Abstract: | Using high-intensity ultrasound, in situ generated α-amylase nanoparticles (NPs) were immobilized on polyethylene (PE) films. The α-amylase NP-coated PE films have been characterized by E-SEM, FTIR, DLS, XPS and RBS. The PE was reacted with HNO3 and NPs of the α-amylase were also deposited on the activated PE. The PE impregnated with α-amylase (4 μg per 1 mg PE) was used for hydrolyzing soluble potato starch to maltose. The immobilization improved the catalytic activity of α-amylase at all the reaction conditions studied. The kinetic parameters, Km (5 and 4 g L?1 for the regular and activated PE, respectively) and Vmax (5 × 10?7 mol ml?1 min?1, almost the same numbers were obtained for the regular and activated PEs) for the immobilized amylase were found to slightly favor the respective values obtained for the free enzyme (Km = 6.6 g L?1, Vmax = 3.7 × 10?7 mol ml?1 min?1). The enzyme remained bound to PE even after soaking the PE in a starch solution for 72 h and was still found to be weakly active. |
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