Structure and interaction potentials in solid-supported lipid membranes studied by X-ray reflectivity at varied osmotic pressure |
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Authors: | U Mennicke D Constantin T Salditt |
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Institution: | 1. Institut für R?ntgenphysik, Friedrich-Hund-Platz 1, 37077, G?ttingen, Germany
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Abstract: | Highly oriented solid-supported lipid membranes in stacks of controlled number N ≃ 16 (oligo-membranes) have been prepared by spin-coating using the uncharged lipid model system 1,2-dimyristoyl-sn-glycero-3-phosphocholine
(DMPC). The samples have been immersed in aqueous polymer solutions for control of osmotic pressure and have been studied
by X-ray reflectivity. The bilayer structure and fluctuations have been determined by modelling the data over the full q-range. Thermal fluctuations are described using the continuous smectic Hamiltonian with the appropriate boundary conditions
at the substrate and at the free surface of the stack. The resulting fluctuation amplitudes and the pressure-distance relation
are discussed in view of the inter-bilayer potential. |
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Keywords: | 61 10 Kw X-ray reflectometry (surfaces interfaces films) 87 16 Dg Membranes bilayers and vesicles 87 15 Ya Fluctuations |
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