Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells |
| |
Authors: | G Caracciolo D Pozzi R Caminiti A Congiu Castellano |
| |
Institution: | (1) Dipartimento di Fisica and INFM, Università “La Sapienza”, P.le A. Moro 5, 00185 Roma, Italy, IT;(2) Dipartimento di Fisica and INFM, Università “Roma Tre”, Via della Vasca Navale 84, 00146 Roma, Italy, IT;(3) Dipartimento di Chimica and INFM, Università “La Sapienza”, P.le A. Moro 5, 00185 Roma, Italy, IT |
| |
Abstract: | We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic
liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol
(Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural
results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation
between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated
gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly
on the cell properties.
Received: 28 October 2002 / Accepted: 21 March 2003 / Published online: 15 April 2003
RID="a"
ID="a"e-mail: a.congiu@caspur.it |
| |
Keywords: | PACS 87 14 Cc Lipids – 61 10 -i X-ray diffraction and scattering |
本文献已被 PubMed SpringerLink 等数据库收录! |
|