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Structural characterization of a new lipid/DNA complex showing a selective transfection efficiency in ovarian cancer cells
Authors:G Caracciolo  D Pozzi  R Caminiti  A Congiu Castellano
Institution:(1) Dipartimento di Fisica and INFM, Università “La Sapienza”, P.le A. Moro 5, 00185 Roma, Italy, IT;(2) Dipartimento di Fisica and INFM, Università “Roma Tre”, Via della Vasca Navale 84, 00146 Roma, Italy, IT;(3) Dipartimento di Chimica and INFM, Università “La Sapienza”, P.le A. Moro 5, 00185 Roma, Italy, IT
Abstract:We investigated, for the first time, by using Energy Dispersive X-ray Diffraction, the structure of a new ternary cationic liposome formulated with dioleoyl trimethylammonium propane (DOTAP), 1,2-dioleoyl-3-phosphatidylethanolamine (DOPE) and cholesterol (Chol) (DDC) which has been recently found to have a selective high gene transfer ability in ovarian cancer cells. Our structural results provide a further experimental support to the widely accepted statement that there is not a simple and direct correlation between structure and transfection efficiency and that the factors controlling cationic lipid/DNA (CL-DNA) complexes-mediated gene transfer depend not only on the formulations of the cationic liposomes and their thermodynamic phase, but also significantly on the cell properties. Received: 28 October 2002 / Accepted: 21 March 2003 / Published online: 15 April 2003 RID="a" ID="a"e-mail: a.congiu@caspur.it
Keywords:PACS  87  14  Cc Lipids –  61  10  -i X-ray diffraction and scattering
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